Laboratory：Akiruno
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- chromosomal test
- chromosomal test for leukemia/malignant lymphomas
- GATA2-MECOM inv(3) inversion,t(3;3) translocation
Laboratory：Akiruno
○GATA2-MECOM inv(3) inversion,t(3;3) translocation
CODE：0R665 6
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
GATA2-MECOM inv(3) inversion,t(3;3) translocation
bone marrow aspirate
1.0
H00
7-10 FISH(Various) Band
Culture lymphocytes or bone marrow cells, and fix metaphase cells. This method then stains the chromosomes with a dye to create a striped pattern (band), and analyzes the distribution and density of the band.
G-Banding, which performs Giemsa staining after treatment with trypsin solution, C-Banding, which performs Giemsa staining after treatment with HCI, Ba (OH) 2, 2×SCC, these include Q-Banding, which involves staining with quinacrine mustard and then observing with a fluorescence microscope, and high-precision banding, which uses mitotic images from the end of prophase to the beginning of metaphase, and observes a larger number of bands than usual.
other material
COMMENT
Avoid freezing. Acceptable days are Monday through Friday.
About Submission of Chromosome Testing Submit the specimen on the same day after collection.
Collect 1.0 mL of bone marrow fluid aseptically in the container shown below, mix well, and preserve refrigerated. Please submit on the same day.
CONTAINER
H00 旧容器記号 H
保存液入り (容器容量5mL)
内容：RPMI-1640 FBS 硫酸カナマイシン ノボヘパリンNa 炭酸水素Na HEPES
貯蔵方法：凍結
有効期間：色が薄いピンクの状態で使用してください。
(凍結時は淡黄色ですが解凍すると薄いピンク色に戻ります｡)
