adrenocortical hormones and their binding protein

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endocrinology test
adrenocortical hormones and their binding protein

Laboratory：Akiruno

○adrenocortical hormones and their binding protein

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Blood 11-OHCS	Serum 0.5	S09 ↓ A00			2-8	Fluorescence method (De Moor modified method)	Blood collection at 10am 7.0-23.0 (μg/dL)
Cortisol	Plasma 0.5	PN2,PN5 ↓ A00		（21 days）	2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.	6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Cortisol	Serum 0.5	S09 ↓ A00			2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.	6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Urinary cortisol	Urine storage 5	A00		（21 days）	2-6	CLIA CLIA (Chemiluminescent immunoassay) Chemiluminescent immunoassay method After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.	4.3-176(μg/day)
Dehydroepiandrosterone sulfate (DHEA-S)	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	See below
17α -hydroxyprogesterone (Under 4 months old)	Serum 0.6	S09 ↓ A00		（21 days）	3-9	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	2.3 or less （Reference value）（ng/mL）
17α -hydroxyprogesterone (5 months of age or older)	Serum 0.6	S09 ↓ A00		（21 days）	3-9	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	2.3 or less （Reference value）（ng/mL）
Pregnenolone（Suspended beyond orders placed 12-05-2019）	Serum 0.9	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.10-1.00 F0.20-1.50 (ng/mL)
Cortisone（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	(Healthy adults 9am to 12pm) M 14.3 to 35.1 F 10.4 to 35.0 (ng/ml)
Aldosterone（Suspended beyond orders placed 03-31-2021）	Plasma 0.5	PN2,PN5 ↓ A00			2-4	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
17-KS7 Fractionation（Suspended beyond orders placed 12-01-2022）	Urine storage 12.0	U20		（28 days）	6-7	Gas Chromatography-Mass Spectrometry (GC/MS) method (enzyme hydrolysis method)	See below
17-KGS（Suspended beyond orders placed 12-05-2019）	Urine storage 15.0	U00		（1 month）	6-8	Colorimetry Colorimetric method A method in which an analyte is converted into a colored substance　under a certain wavelengths and which color tone is compared with that of a standard solution.	M6.00-18.4 F3.55-11.2 (mg/day)
17-KGS2 fractionation（Suspended beyond orders placed 12-05-2019）	Urine storage 17.0	U00		（1 month）	6-8	Colorimetry Colorimetric method A method in which an analyte is converted into a colored substance　under a certain wavelengths and which color tone is compared with that of a standard solution.	11-DEOXY-17KGS M1.54-3.91 F0.84-2.77 11-OXY-17KGS M3.86-13.8 F3.25-8.10 (mg/day)
Corticosterone（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			8-20	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.38-8.42 F0.21-8.48 (ng/mL)
Androsterone（Suspended beyond orders placed 12-05-2019）	Serum 1.0	S09 ↓ A00			8-20	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.18-0.91 F0.14-1.03 (ng/mL)
17-OH pregnenolone (17α-OH pregnenolone)（Suspended beyond orders placed 12-05-2019）	Serum 0.9	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	(ng/mL)
Aldosterone/renin quantitative ratio（Suspended beyond orders placed 03-31-2021）	Plasma 1.0	PN2,PN5 ↓ A00		（20 days）	2-5	Aldosterone: RIA solid phase method Renin quantitative: RIA solid phase method (IRMA)	See below
Cortisol（Suspended beyond orders placed 03-31-2022）	Urine storage 5.0	U00			2-6	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	11.2-80.3(μg/day)
Aldosterone（Suspended beyond orders placed 03-31-2021）	Serum 0.5	S09 ↓ A00			2-4	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
Aldosterone（Suspended beyond orders placed 03-31-2021）	Urine storage 1.5	U00			3-7	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	10 or less (μg/day)
Aldosterone/renin activity ratio（Suspended beyond orders placed 03-31-2021）	Plasma 0.8	PN2,PN5 ↓ A00		（1 month）	3-5	Aldosterone: RIA solid phase method Renin activity: RIA2 antibody method	See below
11-Deoxycortisol（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.04-1.16 F0.11-0.60 (ng/mL)
Deoxycorticosterone (DOC)（Suspended beyond orders placed 12-05-2019）	Serum 1.7	S09 ↓ A00			8-20	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.08-0.28 F0.03-0.33 (ng/mL)
Androstenedione（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			7-13	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	See below

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Blood 11-OHCS	Serum 0.5	S09 ↓ A00			2-8	Fluorescence method (De Moor modified method)	Blood collection at 10am 7.0-23.0 (μg/dL)
Cortisol	Plasma 0.5	PN2,PN5 ↓ A00		（21 days）	2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.	6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Cortisol	Serum 0.5	S09 ↓ A00			2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.	6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Urinary cortisol	Urine storage 5	A00		（21 days）	2-6	CLIA CLIA (Chemiluminescent immunoassay) Chemiluminescent immunoassay method After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.	4.3-176(μg/day)
Dehydroepiandrosterone sulfate (DHEA-S)	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	See below
17α -hydroxyprogesterone (Under 4 months old)	Serum 0.6	S09 ↓ A00		（21 days）	3-9	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	2.3 or less （Reference value）（ng/mL）
17α -hydroxyprogesterone (5 months of age or older)	Serum 0.6	S09 ↓ A00		（21 days）	3-9	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	2.3 or less （Reference value）（ng/mL）
Pregnenolone（Suspended beyond orders placed 12-05-2019）	Serum 0.9	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.10-1.00 F0.20-1.50 (ng/mL)
Cortisone（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	(Healthy adults 9am to 12pm) M 14.3 to 35.1 F 10.4 to 35.0 (ng/ml)
Aldosterone（Suspended beyond orders placed 03-31-2021）	Plasma 0.5	PN2,PN5 ↓ A00			2-4	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
17-KS7 Fractionation（Suspended beyond orders placed 12-01-2022）	Urine storage 12.0	U20		（28 days）	6-7	Gas Chromatography-Mass Spectrometry (GC/MS) method (enzyme hydrolysis method)	See below
17-KGS（Suspended beyond orders placed 12-05-2019）	Urine storage 15.0	U00		（1 month）	6-8	Colorimetry Colorimetric method A method in which an analyte is converted into a colored substance　under a certain wavelengths and which color tone is compared with that of a standard solution.	M6.00-18.4 F3.55-11.2 (mg/day)
17-KGS2 fractionation（Suspended beyond orders placed 12-05-2019）	Urine storage 17.0	U00		（1 month）	6-8	Colorimetry Colorimetric method A method in which an analyte is converted into a colored substance　under a certain wavelengths and which color tone is compared with that of a standard solution.	11-DEOXY-17KGS M1.54-3.91 F0.84-2.77 11-OXY-17KGS M3.86-13.8 F3.25-8.10 (mg/day)
Corticosterone（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			8-20	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.38-8.42 F0.21-8.48 (ng/mL)
Androsterone（Suspended beyond orders placed 12-05-2019）	Serum 1.0	S09 ↓ A00			8-20	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.18-0.91 F0.14-1.03 (ng/mL)
17-OH pregnenolone (17α-OH pregnenolone)（Suspended beyond orders placed 12-05-2019）	Serum 0.9	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	(ng/mL)
Aldosterone/renin quantitative ratio（Suspended beyond orders placed 03-31-2021）	Plasma 1.0	PN2,PN5 ↓ A00		（20 days）	2-5	Aldosterone: RIA solid phase method Renin quantitative: RIA solid phase method (IRMA)	See below
Cortisol（Suspended beyond orders placed 03-31-2022）	Urine storage 5.0	U00			2-6	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	11.2-80.3(μg/day)
Aldosterone（Suspended beyond orders placed 03-31-2021）	Serum 0.5	S09 ↓ A00			2-4	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
Aldosterone（Suspended beyond orders placed 03-31-2021）	Urine storage 1.5	U00			3-7	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	10 or less (μg/day)
Aldosterone/renin activity ratio（Suspended beyond orders placed 03-31-2021）	Plasma 0.8	PN2,PN5 ↓ A00		（1 month）	3-5	Aldosterone: RIA solid phase method Renin activity: RIA2 antibody method	See below
11-Deoxycortisol（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			8-21	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.04-1.16 F0.11-0.60 (ng/mL)
Deoxycorticosterone (DOC)（Suspended beyond orders placed 12-05-2019）	Serum 1.7	S09 ↓ A00			8-20	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	M0.08-0.28 F0.03-0.33 (ng/mL)
Androstenedione（Suspended beyond orders placed 12-05-2019）	Serum 0.5	S09 ↓ A00			7-13	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	See below

TEST DIRECTORY

○adrenocortical hormones and their binding protein

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