○adrenocortical hormones and their binding protein
TEST NAME
SPECIMEN REQUIREMENT (mL)
CONTAINER
CAP COLOR
STORE TEMPERATURE (STABILITY)
TURNAROUND TIME (DAY)
METHODOLOGY
REFERENCE RANGE (UNIT)
Blood 11-OHCS
Serum
0.5
S09 ↓ A00
2-8
Fluorescence method (De Moor modified method)
Blood collection at 10am 7.0-23.0 (μg/dL)
Cortisol
Plasma
0.5
PN2,PN5 ↓ A00
(21 days)
2-4
ECLIA
ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.
6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Cortisol
Serum
0.5
S09 ↓ A00
2-4
ECLIA
ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.
6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Urinary cortisol
Urine storage
5.0
A00
(21 days)
2-6
CLIA
CLIA (Chemiluminescent immunoassay) A method in which after an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, in which the luminescence intensity of a chemiluminescent substrate is measured.
4.3-176(μg/day)
Dehydroepiandrosterone sulfate (DHEA-S)
Serum
0.5
S09 ↓ A00
2-4
CLEIA
CLEIA (Chemiluminescent enzyme immunoassay) A method in which an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, a chemiluminescent substrate is added and the luminescence intensity is measured.
See below
17α-hydroxyprogesterone (aged 4 months or younger)
Serum
0.6
S09 ↓ A00
(21 days)
3-9
ELISA
ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.
2.3 or less (Reference value)(ng/mL)
17α-Hydroxyprogesterone (over 5 months old)
Serum
0.6
S09 ↓ A00
(21 days)
3-9
ELISA
ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
Colorimetric method A method in which an analyte is converted into a colored substance under a certain wavelengths and which color tone is compared with that of a standard solution.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
(ng/mL)
17-KGS(Suspended beyond orders placed 12-05-2019)
Urine storage
15.0
U00
(1 month)
6-8
Colorimetry
Colorimetric method A method in which an analyte is converted into a colored substance under a certain wavelengths and which color tone is compared with that of a standard solution.
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
(Healthy adults 9am to 12pm) M 14.3 to 35.1 F 10.4 to 35.0 (ng/ml)
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
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