○adrenocortical hormones and their binding protein
TEST NAME
SPECIMEN REQUIREMENT （ｍL）
CONTAINER
CAP COLOR
STORE TEMPERATURE (STABILITY)
TURNAROUND TIME (DAY)
METHODOLOGY
REFERENCE RANGE (UNIT)
Blood 11-OHCS
Serum
0.5
S09 ↓ A00
2-8
Fluorescence method (De Moor modified method)
Blood collection at 10am 7.0-23.0 (μg/dL)
Cortisol
Plasma
0.5
PN2,PN5 ↓ A00
（21 days）
2-4
ECLIA
ECLIA (Electro chemiluminescence immunoassay) Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.
6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Cortisol
Serum
0.5
S09 ↓ A00
2-4
ECLIA
ECLIA (Electro chemiluminescence immunoassay) Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.
6:00 a.m.-10:00 a.m. 7.07-19.6 (μg/dL)
Urinary cortisol
Urine storage
5
A00
（21 days）
2-6
CLIA
CLIA (Chemiluminescent immunoassay) Chemiluminescent immunoassay method After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.
4.3-176(μg/day)
Dehydroepiandrosterone sulfate (DHEA-S)
Serum
0.5
S09 ↓ A00
2-4
CLEIA
CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.
See below
17α -hydroxyprogesterone (Under 4 months old)
Serum
0.6
S09 ↓ A00
（21 days）
3-9
ELISA
ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.
2.3 or less （Reference value）（ng/mL）
17α -hydroxyprogesterone (5 months of age or older)
Serum
0.6
S09 ↓ A00
（21 days）
3-9
ELISA
ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
(Healthy adults 9am to 12pm) M 14.3 to 35.1 F 10.4 to 35.0 (ng/ml)
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
Gas Chromatography-Mass Spectrometry (GC/MS) method (enzyme hydrolysis method)
See below
17-KGS（Suspended beyond orders placed 12-05-2019）
Urine storage
15.0
U00
（1 month）
6-8
Colorimetry
Colorimetric method A method in which an analyte is converted into a colored substance under a certain wavelengths and which color tone is compared with that of a standard solution.
Colorimetric method A method in which an analyte is converted into a colored substance under a certain wavelengths and which color tone is compared with that of a standard solution.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
Anytime 35.7-240 Lying position 29.9-159 Standing position 38.9-307 (pg/mL)
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
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