coagulation- and fibrinolysis-related test

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haematological test
coagulation- and fibrinolysis-related test

Laboratory：Akiruno

○coagulation- and fibrinolysis-related test

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Activated partial thromboplastin time (APTT)	Plasma 0.5	PC2 ↓ A00			2-4	Clotting time measurement method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	24.3-36.0 (seconds)
Prothrombin time (PT)	Plasma 0.5	PC2 ↓ A00			2-4	Clotting time measurement method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	Seconds:10.5-13.5 (seconds) %:70-130 (%) Ratio:0.85-1.15
Fibrinogen (FIB)	Plasma 0.5	PC2 ↓ A00			2-4	Clotting time measurement method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	150-400(mg/dL)
Soluble fibrin monomer complex (SFMC)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	LPIA LPIA (Latex photometric immunoassay) Latex near-infrared immunoturbidimetry An antigen-antibody reaction is performed using latex particles bound with antigen or antibody, and the turbidity of agglutination due to the antigen-antibody reaction is measured using near-infrared A method of measuring transmittance by irradiating external light.	Less than 7 (μg/mL) negative
Fibrin monomer complex quantitative	Plasma 0.5	PC2 ↓ A00		（21 days）	2-4	Latex immunoturbidimetry	6.1 or less (μg/mL)
Quantitative FDP	Plasma 0.3	PC2 ↓ A00		（21 days）	2-4	Latex immunoturbidimetry	4 or less (μg/mL)
Quantitative FDP	Partial urine 0.5	U90 ↓ A00		（21 days）	2-6	LPIA LPIA (Latex photometric immunoassay) Latex near-infrared immunoturbidimetry An antigen-antibody reaction is performed using latex particles bound with antigen or antibody, and the turbidity of agglutination due to the antigen-antibody reaction is measured using near-infrared A method of measuring transmittance by irradiating external light.	100 or less (ng/mL)
D-dimer	Plasma 0.5	PC2 ↓ A00		（21 days）	2-4	Latex immunoturbidimetry	1.0 or less (μg/mL)
Prothrombin fragment F1+2	Plasma 0.3	PC2 ↓ A00		（21 days）	2-5	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	69-229(pmol/L)
Antithrombin III (AT-III)	Plasma 0.5	PC2 ↓ A00		（1 month）	2-4	Chromogenic synthetic substrate method Chromogenic synthetic substrate method A method in which heparin is added to form an AT-III-heparin complex, and its ability to inactivate thrombin is measured using a chromogenic synthetic substrate for thrombin.	79-121(%)
Thrombin/Antithrombin complex III (TAT)	Plasma 0.5	PC2 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	3.0 or less (ng/mL)
Plasminogen	Plasma 0.5	PC2 ↓ A00			2-4	Chromogenic synthetic substrate method Chromogenic synthetic substrate method A method in which heparin is added to form an AT-III-heparin complex, and its ability to inactivate thrombin is measured using a chromogenic synthetic substrate for thrombin.	75-125(%)
Antiplasmin (α₂ plasmin inhibitor)	Plasma 0.5	PC2 ↓ A00		（1 month）	2-4	Chromogenic synthetic substrate method Chromogenic synthetic substrate method A method in which heparin is added to form an AT-III-heparin complex, and its ability to inactivate thrombin is measured using a chromogenic synthetic substrate for thrombin.	85-115(%)
α₂Plasmin inhibitor-plasmin complex (PIC test)	Plasma 0.2	PC2 ↓ A00		（21 days）	2-4	LPIA LPIA (Latex photometric immunoassay) Latex near-infrared immunoturbidimetry An antigen-antibody reaction is performed using latex particles bound with antigen or antibody, and the turbidity of agglutination due to the antigen-antibody reaction is measured using near-infrared A method of measuring transmittance by irradiating external light.	0.8 or less (μg/mL)
Total PAI-1 (tPA/PAI-1 complex)	Plasma 0.5	PC2 ↓ A00		（21 days）	2-5	LPIA LPIA (Latex photometric immunoassay) Latex near-infrared immunoturbidimetry An antigen-antibody reaction is performed using latex particles bound with antigen or antibody, and the turbidity of agglutination due to the antigen-antibody reaction is measured using near-infrared A method of measuring transmittance by irradiating external light.	50 or less (ng/mL)
Coagulation factor activity test Factor VIII (F8) 〔synthetic substrate method〕	Plasma 0.3	PC2 ↓ A00		（7 days）	2-6	Synthetic substrate method Chromogenic synthetic substrate method A method in which heparin is added to form an AT-III-heparin complex, and its ability to inactivate thrombin is measured using a chromogenic synthetic substrate for thrombin.	60-140(%)
Coagulation factor activity test Factor IX (F9) 〔synthetic substrate method〕	Plasma 0.3	PC2 ↓ A00		（28 days）	2-6	Synthetic substrate method Chromogenic synthetic substrate method A method in which heparin is added to form an AT-III-heparin complex, and its ability to inactivate thrombin is measured using a chromogenic synthetic substrate for thrombin.	60-140(%)
Coagulation factor activity test Factor II (F2)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	75-135(%)
Coagulation factor activity test Factor V (F5)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	70-135(%)
Coagulation factor activity test Factor VII (F7)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	75-140(%)
Coagulation factor activity test Factor VIII (F8)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	60-150(%)
Coagulation factor activity test Factor IX (F9)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	70-130(%)
Coagulation factor activity test Factor X (F10)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	70-130(%)
Coagulation factor activity test Factor XI (F11)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	75-145(%)
Coagulation factor activity test Factor XII (F12)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	50-150(%)
Coagulation factor activity test Factor ⅩⅢ (F13)	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	Synthetic substrate method Chromogenic synthetic substrate method A method in which heparin is added to form an AT-III-heparin complex, and its ability to inactivate thrombin is measured using a chromogenic synthetic substrate for thrombin.	70-140(%)
Von Willbrand factor antigen quantitative	Plasma 0.2	PC2 ↓ A00		（21 days）	2-4	latex agglutination reaction LA (Latex agglutination) Latex agglutination A method to determine the presence of antibody or antigen by antigen-antibody reaction using latex particles (sensitized latex particles) to which antigen or antibody is adsorbed (bound) and by the presence or absence of agglutination by antigen-antibody reaction.	50-155(%)
Coagulation inhibitor identification Factor VIII	Plasma 1.0	PC5 ↓ A00			2-4	Bethesda Act	Not detected (Bethesda U/mL)
Coagulation inhibitor identification Factor IX	Plasma 1.0	PC5 ↓ A00		（21 days）	2-4	Bethesda Act	Not detected (Bethesda U/mL)
Von-Wilbrand factor activity (Ristocetin cofactor)	Plasma 0.5	PC2 ↓ A00		（20 days）	2-4	Fixed platelet aggregation method	60-170(%)
Von-Wilbrand factor multimer analysis	Plasma 0.4	PC2 ↓ A00		（1 month）	7-14	SDS agarose gel electrophoresis method Electrophoresis method A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.	NORMAL pattern
ADAMTS13 activity	Plasma 0.3	PC2 ↓ A00		（28 days）	2-5	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	0.10 or more (IU/mL) 10 or more (%)
ADAMTS13 inhibitor	Plasma 0.6	PC2 ↓ A00		（28 days）	2-5	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 0.5 (BU/mL) Negative
β-thromboglobulin (β-TG)	Platelet-poor plasma 0.3	PBT ↓ A00		（1 month）	2-9	Enzyme immunoassay(EIA) EIA (Enzyme immunoassay) Enzyme immunoassay The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.	50 or less (ng/mL)
Platelet factor-4 (PF-4)	Platelet-poor plasma 0.3	PBT ↓ A00		（1 month）	2-9	Enzyme immunoassay(EIA) EIA (Enzyme immunoassay) Enzyme immunoassay The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.	20 or less (ng/mL)
Protein C (antigen level)	Plasma 0.3	PC2 ↓ A00			2-5	LPIA LPIA (Latex photometric immunoassay) Latex near-infrared immunoturbidimetry An antigen-antibody reaction is performed using latex particles bound with antigen or antibody, and the turbidity of agglutination due to the antigen-antibody reaction is measured using near-infrared A method of measuring transmittance by irradiating external light.	70-150(%)
Protein C activity	Plasma 0.4	PC2 ↓ A00			2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	64-146(%)
Protein S (antigen level)	Plasma 0.2	PC2 ↓ A00		（21 days）	2-4	latex agglutination reaction LA (Latex agglutination) Latex agglutination A method to determine the presence of antibody or antigen by antigen-antibody reaction using latex particles (sensitized latex particles) to which antigen or antibody is adsorbed (bound) and by the presence or absence of agglutination by antigen-antibody reaction.	M73-137 F59-143(%)
Protein S activity	Plasma 0.4	PC2 ↓ A00			2-4	Clotting time method Clotting time Clotting time is the time required for a plasma sample to coagulate, using plasma without a factor to be tested, thromboplastin, actin, and calcium chloride.	M67-164 F56-126(%)
Protein S (free antigen level)	Plasma 0.2	PC2 ↓ A00		（21 days）	2-4	latex agglutination reaction LA (Latex agglutination) Latex agglutination A method to determine the presence of antibody or antigen by antigen-antibody reaction using latex particles (sensitized latex particles) to which antigen or antibody is adsorbed (bound) and by the presence or absence of agglutination by antigen-antibody reaction.	M50-131 F49-133(%)
Thrombomodulin	Serum 0.4	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	12.1-24.9(U/mL)
Thrombomodulin	Plasma 0.4	PC2 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	8.7-22.7(U/mL)
Thrombin-antithrombin III complex (TAT)（Suspended beyond orders placed 12-01-2017）	Plasma 0.5	PC2 ↓ A00				Enzyme immunoassay(EIA) EIA (Enzyme Immunoassay Assay) Enzyme immunoassay The principle of measurement is the same as that of RIA. An antigen-antibody reaction is performed using an antigen or antibody labeled with an enzyme to a labeled substance, and a chromogenic substrate is added to measure enzyme activity.	3.0 or less (ng/mL)
Thrombotest (TT)（Suspended beyond orders placed 03-31-2021）	Plasma 0.5	PC2 ↓ A00			2-4	Clotting time measurement method Clotting time method A method in which plasma deficient in the factor to be measured is added with thromboplastin, actin, and calcium chloride, and in which the time until coagulation is measured.	70 or more (%)
Hepaplastin Test (HPT)（Suspended beyond orders placed 12-05-2019）	Plasma 0.5	PC2 ↓ A00			2-4	Clotting time measurement method Clotting time method A method in which plasma deficient in the factor to be measured is added with thromboplastin, actin, and calcium chloride, and in which the time until coagulation is measured.	70-130(%)

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○coagulation- and fibrinolysis-related test

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