TEST DIRECTORY

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Laboratory：Akiruno

○lipids and related materials

TEST NAME

 SPECIMEN
REQUIREMENT
（ｍL）		 CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY)		 TURNAROUND
TIME (DAY)		 METHODOLOGY REFERENCE RANGE
(UNIT)

Triglycerides (TG)

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 Enzymatic method(GK-GPO free glycerol blank)

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 50-149(mg/dL)

Phospholipid

Serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 160-260(mg/dL)

Free (or non-esterified) fatty acid(NEFA)

Serum
0.3
S09
↓
A00 		Freeze
（28 days）
 2-4 Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 140-850(μEq/L)

Total cholesterol (T-Cho)

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 Cholesterol oxidase (COD-POD) method 150-219(mg/dL)

Esterified cholesterol (Echo)

Serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 Cholesterol oxidase(COD-POD) method, Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 90-200(mg/dL)

Free cholesterol (F-Cho)

Serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 30-60(mg/dL)

Cholesterol ester rate

Serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 Cholesterol oxidase(COD-POD) method, Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 73-77(%)

HDL-cholesterol

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 direct method M 40-86
F 40-96
(mg/dL)

HDL2, 3-cholesterol

Serum
1.0
S09
↓
A00 		Refrigeration
（20 days）
 2-6 ultracentrifugation method

Ultracentrifugation method
A method of separating and measuring proteins based on the difference in their specific gravity using an ultracentrifuge.

 HDL2 Cholesterol
M 16.0-61.0
F 23.0-73.0
HDL3 Cholesterol
M 13.0-25.0
F 13.0-24.0
(mg/dL)

LDL-cholesterol

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 direct method 70-139(mg/dL)

Remnant-like particles-cholesterol (RLP-C)

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 7.5 or less (mg/dL)

sd LDL-cholesterol

Serum
0.5
S09
↓
A00 		Refrigeration
（5 days）
 2-4 direct method Less than 35.0 (mg/dL)

Oxidized LDL (MDA-LDL)

Serum
0.3
S09
↓
A00 		Freeze
（21 days）
 3-14 ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 Male
Under 45 years old 46-82
Over 45 years old 61-105
Female
Under 55 years old 46-82
Over 55 years old 61-105
(U/L)

sd LDL-C (physical examination)

Serum
0.5
S09
↓
A00 		Refrigeration
（5 days）
 2-4 direct method (mg/dL)

Fatty acid fractionation (24)

Plasma
0.5
PH5
↓
A00 		Refrigeration
（45 days）
 10-14 Gas-Chromatograph method

Chromatography
A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components.
When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.

 See below

Fatty acid fractionation (24)

Serum
0.5
S09
↓
A00 		Refrigeration
（45 days）
 10-14 Gas-Chromatograph method

Chromatography
A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components.
When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.

 See below

Fatty acid fractionation (4)

Plasma
0.5
PH5
↓
A00 		Refrigeration
（45 days）
 4-6 Gas-Chromatograph method

Chromatography
A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components.
When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.

 Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61 DHA/AA ratio 0.27-1.07 (EPA +DHA)/ AA ratio 0.32-1.66

Fatty acid fractionation (4)

Serum
0.5
S09
↓
A00 		Refrigeration
（45 days）
 4-6 Gas-Chromatograph method

Chromatography
A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components.
When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.

 Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61 DHA/AA ratio 0.27-1.07 (EPA +DHA)/ AA ratio 0.32-1.66

Very long chain fatty acid

Plasma
2.0
PH5
↓
A00 		Freeze
（1 month）
 6-19 Gas chromatography method (GC-MS)

Chromatography
A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components.
When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.

 C24:0/C22:0
0.628-0.977
C25:0/C22:0
0.012-0.023
C26:0/C22:0
0.003-0.006

Total bile acids

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 10.0 or less (μmol/L)

β-lipoprotein

Serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 220-650(mg/dL)

Lipoprotein fractionation

Serum
0.2
S09
↓
A00 		Refrigeration
（7 days）
 2-4 Agarose gel electrophoresis

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

 α
M26.9-50.5
F32.6-52.5
PREβ
M7.9-23.8
F6.6-20.8
β
M35.3-55.5
F33.6-52.0
(%)

Cholesterol fractionation

Serum
0.2
S09
↓
A00 		Refrigeration
（7 days）
 2-4 Agarose gel electrophoresis

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

 HDL cholesterol 23-48
LDL cholesterol 47-69
VLDL cholesterol 2-15
(%)

Lipoprotein (a)

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 LA (latex agglutination turbidimetry)

LA (Latex agglutination immunoassay)
Latex agglutination turbidimetry
An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.

 40 or less (Reference value)(mg/dL)

Lipoprotein lipase (LPL)

Immediately Refrigerated
Plasma
0.3
PH5
↓
A00 		Freeze
（1 month）
 2-8 ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 164-284(ng/mL)

Apolipoprotein A-I

Serum
0.3
S09
↓
A00 		Refrigeration
（1 month）
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 M 119-155
F 126-165
(mg/dL)

Apolipoprotein A-II

Serum
0.3
S09
↓
A00 		Refrigeration
（1 month）
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 M 25.9-35.7
F 24.6-33.3
(mg/dL)

Apolipoprotein B

Serum
0.3
S09
↓
A00 		Refrigeration
（1 month）
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 M 73-109
F 66-101
(mg/dL)

Apolipoprotein C-II

Serum
0.3
S09
↓
A00 		Refrigeration
（1 month）
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 M 1.8-4.6
F 1.5-3.8
(mg/dL)

Apolipoprotein C-III

Serum
0.3
S09
↓
A00 		Refrigeration
（1 month）
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 M 5.8-10.0
F 5.4-9.0
(mg/dL)

Apolipoprotein E

Serum
0.3
S09
↓
A00 		Refrigeration
（1 month）
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 M 2.7-4.3
F 2.8-4.6
(mg/dL)

Pulmonary surfactant protein A (SP-A)

Serum
0.3
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 43.8 (ng/mL)

Pulmonary surfactant protein D (SP-D)

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 110 (ng/mL)

INACTIVE
Lipoprotein lipid fraction〔
Lipoprotein cholesterol fraction quantitation
Lipoprotein trigLipoprotein triglyceride fraction determination
Lipoprotein phospholipid fraction determination〕（Suspended beyond orders placed 12-03-2020）

Serum
1.0
S09
↓
A00 		Refrigeration
 2-4 ultracentrifugation method

Ultracentrifugal method
A method of separating and measuring proteins by the difference in specific gravity using an ultracentrifuge.

 See below

INACTIVE
Lipoprotein Cholesterol (LP-C) Fractional Quantitation（Suspended beyond orders placed 12-03-2020）

Serum
1.0
S09
↓
A00 		Refrigeration
（20 days）
 2-4 ultracentrifugation method

Ultracentrifugal method
A method of separating and measuring proteins by the difference in specific gravity using an ultracentrifuge.

 See below

INACTIVE
Fatty acid fraction of total lipids（Suspended beyond orders placed 12-29-2017）

Serum
0.5
S09
↓
A00 		Freeze
（1 month）
 10-14 Gas-Chromatograph method

Chromatography
A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases.
It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.

 See below

INACTIVE
Apolipoprotein E phenotype（Suspended beyond orders placed 10-03-2024）

Serum
0.2
S09
↓
A00 		Refrigeration
（7 days）
 4-20 Immunoblot method using isoelectric focusing method

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

INACTIVE
Free fatty acids(FFA，NEFA)（Suspended beyond orders placed 06-30-2016）

Serum
0.3
S09
↓
A00 		Freeze
（1 month）
 2-4 Enzymatic method

Enzyme method
The measurement principle is the same as the colorimetric method, and the measurement substance is specifically measured using an enzyme.

 140-850(μEq/L)

INACTIVE
Blood fatty acid fraction（Suspended beyond orders placed 12-29-2017）

Plasma
0.5
PH5
↓
A00 		Freeze
（35 days）
 4-6 Gas-Chromatograph method

Chromatography
A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases.
It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.

 Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61

INACTIVE
L-CAT（Suspended beyond orders placed 03-31-2016）

Serum
1.5
S09
↓
A00 		Refrigeration
 Dipalmitoyl lecithin substrate method

INACTIVE
Fatty acid fraction of total lipids（Suspended beyond orders placed 12-29-2017）

Plasma
0.5
PH5
↓
A00 		Freeze
（1 month）
 10-14 Gas-Chromatograph method

Chromatography
A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases.
It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.

 See below

INACTIVE
Blood fatty acid fraction（Suspended beyond orders placed 12-29-2017）

Serum
0.5
S09
↓
A00 		Freeze
（35 days）
 4-6 Gas-Chromatograph method

Chromatography
A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases.
It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.

 Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61

INACTIVE
Lipoprotein fraction（Suspended beyond orders placed 09-30-2021）

Serum
0.5
S09
↓
A00 		Refrigeration
（7 days）
 3-7 Polyacrylamide gel disk electrophoresis method

Electrophoresis
A method using the phenomenon that, when electricity is applied to an electrolyte solution with suspended charged particles, the particles migrate toward the polarity opposite to the charge on each particle, to measure the analyte based on its mobility.
For the aqueous solution support, cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used.

 HDL
M22-47
F27-50
LDL
M46-68
F44-66
VLDL
M3-19
F2-12
(%)

INACTIVE
Glycolic acid(CG)（Suspended beyond orders placed 03-31-2022）

Serum
0.2
S09
↓
A00 		Refrigeration
 2-8 RIA PEG method

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

 50 or less (μg/dL)

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