*Lymphocyte surface marker automate analysis by monoclonal antibodies
Laboratory:Akiruno
○*Lymphocyte surface marker automate analysis by monoclonal antibodies
TEST NAME
SPECIMEN REQUIREMENT (mL)
CONTAINER
CAP COLOR
STORE TEMPERATURE (STABILITY)
TURNAROUND TIME (DAY)
METHODOLOGY
REFERENCE RANGE (UNIT)
CD1a
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
1.0 or less (%)
CD2
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
71.0-91.0(%)
CD3
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
58.0-84.0(%)
CD4
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
25.0-54.0(%)
CD8
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
23.0-56.0(%)
CD4/CD8 ratio
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
0.40-2.30
CD5
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
60.0-90.0(%)
CD7
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
72.0-90.0(%)
CD10
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
1.0 or less (%)
CD11b
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
18.0-49.0(%)
CD13
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
9.0 or less (%)
CD14
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
5.0 or less (%)
CD16
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
6.0-39.0(%)
CD19
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
5.0-24.0(%)
CD20
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
3.0-20.0(%)
CD21
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
3.0-18.0(%)
CD22
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
2.0-17.0(%)
CD25
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
8.0-34.0(%)
CD30
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
1.0 or less (%)
CD33
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
5.0 or less (%)
CD34
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
1.0 or less (%)
CD38
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
18.0-59.0(%)
CD56
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
9.0-43.0(%)
CD57
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
4.0-45.0(%)
HLA-DR
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
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