*Lymphocyte surface marker automate analysis by monoclonal antibodies
Laboratory:Akiruno
○*Lymphocyte surface marker automate analysis by monoclonal antibodies
TEST NAME
SPECIMEN REQUIREMENT (mL)
CONTAINER
CAP COLOR
STORE TEMPERATURE (STABILITY)
TURNAROUND TIME (DAY)
METHODOLOGY
REFERENCE RANGE (UNIT)
CD1a
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
1.0 or less (%)
CD2
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
71.0-91.0(%)
CD3
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
58.0-84.0(%)
CD4
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
25.0-54.0(%)
CD8
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
23.0-56.0(%)
CD4/CD8 ratio
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
0.40-2.30
CD5
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
60.0-90.0(%)
CD7
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
72.0-90.0(%)
CD10
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
1.0 or less (%)
CD11b
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
18.0-49.0(%)
CD13
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
9.0 or less (%)
CD14
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
5.0 or less (%)
CD16
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
6.0-39.0(%)
CD19
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
5.0-24.0(%)
CD20
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
3.0-20.0(%)
CD21
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
3.0-18.0(%)
CD22
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
2.0-17.0(%)
CD25
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
8.0-34.0(%)
CD30
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
1.0 or less (%)
CD33
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
5.0 or less (%)
CD34
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
1.0 or less (%)
CD38
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
18.0-59.0(%)
CD56
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
9.0-43.0(%)
CD57
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
4.0-45.0(%)
HLA-DR
Blood (heparin added)
3.0
PH5
3-5
Flow cytometry(FCM)
Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
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