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autoimmune-related test

Laboratory：Akiruno

○autoimmune-related test

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Anti-nuclear antibody (ANA)	Serum 0.3	S09 ↓ A00			2-4	FA FA (Fluorescent antibody method) Fluorescent antibody method A method in which an antigen-antibody reaction is performed against the target antigen using an antibody labeled with a fluorescent dye, and the fluorescence intensity is measured under a fluorescence microscope. There is a direct method in which an antibody labeled with a fluorescent dye is reacted directly, and an indirect method in which the antibody is reacted with an antigen and then a secondary reaction is performed with an antibody labeled with a fluorescent dye.	Less than 40 (X)
Anti-DNA antibody〔RIA〕	Serum 0.2	S09 ↓ A00			2-4	RIA ammonium sulfate salting out method RIA (Radio immunoassay) Radioimmunoassay A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity. Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	6.0 or less (IU/mL)
Anti-ss-DNA IgG antibody	Serum 0.3	S09 ↓ A00			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	25 or less (AU/mL)
Anti-ss-DNA IgM antibody	Serum 0.3	S09 ↓ A00			2-15	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 17(U/mL)
Anti-ds-DNA IgG antibody	Serum 0.3	S09 ↓ A00			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	12 or less (IU/mL)
Anti-ds-DNA IgM antibody	Serum 0.3	S09 ↓ A00			2-15	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 6 (U/mL)
Anti-Sm antibody	Serum 0.5	S09 ↓ A00			3-5	Ochterlony method Ouchterlony method In-plate double immunodiffusion method One of the in-gel diffusion methods called in-plate double immunodiffusion method. A method in which the antibody and antigen are diffused in a gel, and the reaction between the antigen and antibody is confirmed by the number of sedimentation lines formed by the antigen-antibody reaction and the presence or absence of reactivity.	Negative (X)
Anti-Sm antibody〔CLEIA〕	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 10.0 (U/mL)
Anti-RNP antibody	Serum 0.5	S09 ↓ A00			3-5	Ochterlony method Ouchterlony method In-plate double immunodiffusion method One of the in-gel diffusion methods called in-plate double immunodiffusion method. A method in which the antibody and antigen are diffused in a gel, and the reaction between the antigen and antibody is confirmed by the number of sedimentation lines formed by the antigen-antibody reaction and the presence or absence of reactivity.	Negative (X)
Anti-RNP antibody〔CLEIA〕	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 10.0 (U/mL)
Anti-SS-A/Ro antibody	Serum 0.5	S09 ↓ A00			3-5	Ochterlony method Ouchterlony method In-plate double immunodiffusion method One of the in-gel diffusion methods called in-plate double immunodiffusion method. A method in which the antibody and antigen are diffused in a gel, and the reaction between the antigen and antibody is confirmed by the number of sedimentation lines formed by the antigen-antibody reaction and the presence or absence of reactivity.	Negative (X)
Anti-SS-A/Ro antibody〔CLEIA〕	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 10.0 (U/mL)
Anti-SS-B/La antibody	Serum 0.5	S09 ↓ A00			3-5	Ochterlony method Ouchterlony method In-plate double immunodiffusion method One of the in-gel diffusion methods called in-plate double immunodiffusion method. A method in which the antibody and antigen are diffused in a gel, and the reaction between the antigen and antibody is confirmed by the number of sedimentation lines formed by the antigen-antibody reaction and the presence or absence of reactivity.	Negative (X)
Anti-SS-B/La antibody〔CLEIA〕	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 10.0 (U/mL)
Anti-Scl-70 antibody	Serum 0.5	S09 ↓ A00			3-5	Ochterlony method Ouchterlony method In-plate double immunodiffusion method One of the in-gel diffusion methods called in-plate double immunodiffusion method. A method in which the antibody and antigen are diffused in a gel, and the reaction between the antigen and antibody is confirmed by the number of sedimentation lines formed by the antigen-antibody reaction and the presence or absence of reactivity.	Negative (X)
Anti-Scl-70 antibody〔CLEIA〕	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 10.0 (U/mL)
Anti-RNA polymerase III antibody	Serum 0.3	S09 ↓ A00			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 28 Criteria: See below
Anti-centromere antibody	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 10.0 (U/mL)
Anti-Jo-1 antibody	Serum 0.3	S09 ↓ A00			3-5	Ochterlony method Ouchterlony method In-plate double immunodiffusion method One of the in-gel diffusion methods called in-plate double immunodiffusion method. A method in which the antibody and antigen are diffused in a gel, and the reaction between the antigen and antibody is confirmed by the number of sedimentation lines formed by the antigen-antibody reaction and the presence or absence of reactivity.	Negative (X)
Anti-Jo-1 antibody〔CLEIA〕	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 10.0 (U/mL)
Anti-ARS antibody	Serum 0.3	S09 ↓ A00		（28 days）	2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 25.0 Negative
Anti MDA5 antibody	Serum 0.3	S09 ↓ A00		（28 days）	2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 32 negative
Anti Mi-2 antibody	Serum 0.3	S09 ↓ A00		（28 days）	2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 53 negative
Anti-TIF1-γ antibody	Serum 0.3	S09 ↓ A00		（28 days）	2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 32 negative
Rheumatoid factor (RF) Quantitative	Serum 0.5	S09 ↓ A00			2-4	Latex agglutination immunoturbidimetry	15 or less (IU/mL)
IgG rheumatoid factor	Serum 0.3	S09 ↓ A00			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 2.00
Anti-galactose deficient IgG antibody	Serum 0.3	S09 ↓ A00			2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.	Less than 6.0 (AU/mL)
Anti-cyclic citrullinated peptide (CCP) antibody	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 4.5 (U/mL)
Anti-mitochondrial antibody	Serum 0.3	S09 ↓ A00			2-4	FA FA (Fluorescent antibody method) Fluorescent antibody method A method in which an antigen-antibody reaction is performed against the target antigen using an antibody labeled with a fluorescent dye, and the fluorescence intensity is measured under a fluorescence microscope. There is a direct method in which an antibody labeled with a fluorescent dye is reacted directly, and an indirect method in which the antibody is reacted with an antigen and then a secondary reaction is performed with an antibody labeled with a fluorescent dye.	Negative (less than 20) (X)
Anti-mitochondrial M2 antibody	Serum 0.5	S09 ↓ A00		（28 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 7.0
Anti-smooth muscle antibody	Serum 0.3	S09 ↓ A00			2-4	FA FA (Fluorescent antibody method) Fluorescent antibody method A method in which an antigen-antibody reaction is performed against the target antigen using an antibody labeled with a fluorescent dye, and the fluorescence intensity is measured under a fluorescence microscope. There is a direct method in which an antibody labeled with a fluorescent dye is reacted directly, and an indirect method in which the antibody is reacted with an antigen and then a secondary reaction is performed with an antibody labeled with a fluorescent dye.	Negative (less than 40) (X)
Anti-gastric wall cell antibody	Serum 0.3	S09 ↓ A00			2-4	FA FA (Fluorescent antibody method) Fluorescent antibody method A method in which an antigen-antibody reaction is performed against the target antigen using an antibody labeled with a fluorescent dye, and the fluorescence intensity is measured under a fluorescence microscope. There is a direct method in which an antibody labeled with a fluorescent dye is reacted directly, and an indirect method in which the antibody is reacted with an antigen and then a secondary reaction is performed with an antibody labeled with a fluorescent dye.	Negative (less than 10) (X)
Anti-intrinsic factor antibody	Serum 0.8	S09 ↓ A00			2-6	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	(-)
Fecal calprotectin	Feces	F30		（28 days）	3-7	FEIA FEIA (Fluorescence enzyme immunoassay) Fluorescence enzyme immunoassay A type of EIA, an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a fluorescent substrate is added to increase the fluorescence intensity. How to measure.	50.0 or less Cutoff value for endoscopic inactivity in ulcerative colitis 300 or less Cutoff value for endoscopic inactivity in Crohn's disease 80 or less (mg/kg)
Fecal calprotectin	Feces 1g	F00			5-9	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 94.0 Cutoff value for understanding the pathology of ulcerative colitis Less than 240 Cutoff value for understanding the pathology of Crohn's disease 80 or less (μg/g)
Leucine-rich α2 glycoprotein (LRG)	Serum 0.5	S09 ↓ A00		（28 days）	2-4	Latex agglutination immunoassay	Less than 16.0 (cutoff value) (μg/mL)
Anti-thyroid peroxidase antibody (anti-TPO antibody)〔CLEIA〕	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 3.3 (IU/mL)
Anti-thyroglobulin antibody〔CLEIA〕	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 19.3 (IU/mL)
Thyroid stimulating antibody (TSAb)	Serum 0.3	S09 ↓ A00		（21 days）	2-4	Bioassay	Less than 110（%）
TSH receptor antibody (quantitative)	Serum 0.4	S09 ↓ A00			2-5	RRA RRA (Radio receptor assay) Radio receptor assay The measurement principle is the same as RIA, a method that uses a receptor instead of an antibody and determines biological activity from its reactivity.	Less than 1.0 (IU/L)
TSH receptor antibody〔CLEIA〕	Serum 0.8	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 2.0 (IU/L)
Anti-GAD antibody	Serum 0.3	S09 ↓ A00			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 5.0 (U/mL)
Anti-IA-2 antibody	Serum 0.6	S09 ↓ A00		（28 days）	3-9	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 0.6 (U/mL)
Zinc transporter 8 (ZnT8) antibody	Serum 0.3	S09 ↓ A00		（28 days）	Please contact us in advance.	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 15.0(U/mL)
Insulin antibody	Serum 0.3	S09 ↓ A00			3-7	RIA RIA (Radio immunoassay) Radioimmunoassay A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity. Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	Less than 0.4 (U/mL)
Anti-acetylcholine receptor antibody (anti-AChR antibody)	Serum 0.3	S09 ↓ A00			3-7	RIA RIA (Radio immunoassay) Radioimmunoassay A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity. Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	0.2 or less (nmol/L)
Anti-muscle specific tyrosine kinase antibody (anti-MuSK antibody)	Serum 0.3	S09 ↓ A00		（21 days）	3-9	RIA RIA (Radio immunoassay) Radioimmunoassay A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity. Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	Less than 0.02 (nmol/L)
Anti-P/Q type potential-dependent calcium channel antibody (anti-P/Q type VGCC antibody)	Serum 0.3	S09 ↓ A00		（28 days）	2-15	RIA RIA (Radio immunoassay) Radioimmunoassay A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity. Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	Less than 30.0 (pmol/L)
Anti-desmoglein 1 antibody	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 20.0 (U/mL)
Anti-desmoglein 3 antibody	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 20.0 (U/mL)
Anti-BP180 antibody (serum anti-BP180NC16a antibody)	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 9.0 U/mL)
Anti-glomerular basement membrane antibody (anti-GBM antibody)	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 3.0 (U/mL)
Sperm immobilizing antibody	Serum 0.3	S09 ↓ A00		（9 months）	Please contact us in advance.	Immobilization method (Isojima method)	(-) criteria: See below
Sperm immobilizing antibody(SI50)	Serum 0.3	S09 ↓ A00		（9 months）	Please contact us in advance.	Immobilization method (Isojima method)	less than 1.0
Lupus anticoagulant	Plasma 1.0	PC5 ↓ A00			2-4	APTT coagulation time method	46.5 or less (seconds)
Lupus anticoagulant	Plasma 1.0	PC5 ↓ A00		（21 days）	2-4	Dilute Russell's snake venom time method	1.2 or less
Lupus anticoagulant	Plasma 1.0	PC5 ↓ A00			2-4	Phospholipid neutralization method	1.16 or less
Anti-cardiolipin β₂glycoprotein I complex antibody (anti-CL/β₂GPⅠ antibody)	Serum 0.3	S09 ↓ A00		（1 month）	2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 3.5 (U/mL)
Anti-β₂ glycoprotein I IgG antibody	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 0.7 (U/mL)
Anti-β₂ glycoprotein I IgM antibody	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	17.5 or less (U/mL)
Anti-cardiolipin IgG antibody	Serum 0.3	S09 ↓ A00		（7 days）	2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	12.3 or less (U/mL)
Anti-cardiolipin IgM antibody	Serum 0.3	S09 ↓ A00		（14 days）	2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	20.8 or less (U/mL)
Anti-platelet antibody	Serum 0.4	S09 ↓ A00			3-6	MPHA (mixed passive aggregation method)	Negative
PAIgG (platelet-associated IgG)	Blood (ACD-A liquid added) 7.5	PAC			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	46 or less (ng/10⁷cells)
HIT antibody (platelet factor IV/heparin complex antibody)	Plasma 0.5	PC2 ↓ A00			2-6	Latex turbidimetry	Less than 1.0 Negative (U/mL)
Anti-Neutrophil Cytoplasmic Antibody (PR3-ANCA)	Serum 0.5	S09 ↓ A00		（28 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 3.5 (U/mL)
Anti-Neutrophil Cytoplasmic Antibody (MPO-ANCA)	Serum 0.5	S09 ↓ A00		（28 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 3.5 (U/mL)
Anti-LKM-1 antibody	Serum 0.3	S09 ↓ A00			2-8	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 17 criteria: See below
Immune complex (monoclonal RF)	Serum 0.2	S09 ↓ A00		（21 days）	2-8	Enzyme immunoassay(EIA) EIA (Enzyme immunoassay) Enzyme immunoassay The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.	Less than 4.2 (μg/mL)
Anti-aquaporin 4 antibody	Serum 0.4	S09 ↓ A00		（28 days）	2-8	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Less than 3.0 Negative (U/mL)
Anti-epidermal component autoantibodies 〔direct method〕	Skin tissue 5×5×3mm	VP1			12-14	Fluorescent antibody staining method
Anti-thyroglobulin antibody（Suspended beyond orders placed 09-30-2021）	Serum 0.5	S09 ↓ A00			2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.	Less than 28(IU/mL)
Anti-cardiolipin antibody (IgG)（Suspended beyond orders placed 03-31-2022）	Serum 0.3	S09 ↓ A00			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	Less than 10 (U/mL)
TSH-stimulating receptor antibody (TSAb)（Suspended beyond orders placed 03-31-2023）	Serum 0.8	S09 ↓ A00		（21 days）	3-5	BioassayEIA	120 or less (%)
Anti-cardiolipin antibody (IgM)（Suspended beyond orders placed 03-31-2022）	Serum 0.3	S09 ↓ A00			3-5	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	Less than 8 (U/mL)
TSH Receptor Antibody（Suspended beyond orders placed 03-31-2021）	Serum 0.2	S09 ↓ A00			2-4	RRA RRA (Radio receptor assay) Radio receptor assay A method similar to RIA in measurement principle, using receptors instead of antibodies to determine biological activity from their reactivity.	15 or less (%)
TSH receptor antibody (3rd generation)（Suspended beyond orders placed 09-30-2021）	Serum 0.5	S09 ↓ A00			2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.	Less than 2.0 (IU/L)
Anti-aquaporin 4 antibody（Suspended beyond orders placed 04-03-2017）	Serum 0.4	S09 ↓ A00				ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	Less than 5.0 (U/mL)
Lupus anti-core grant（Suspended beyond orders placed 03-31-2016）	Plasma 1.0	PC5 ↓ A00				Phospholipid neutralization method	6.3 or less (seconds)
Anti-IA-2 antibody（Suspended beyond orders placed 12-06-2018）	Serum 0.3	S09 ↓ A00			3-9	RIA RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	Less than 0.4 (reference cutoff value 1.0) (U/mL)
Anti-pituitary antibody 1（Suspended beyond orders placed 09-29-2020）	Serum 0.9	S09 ↓ A00			7-13	Indirect fluorescent antibody method	(-)
Anti-thyroid microsomal antibody (microsome test)（Suspended beyond orders placed 12-01-2022）	Serum 0.3	S09 ↓ A00			2-4	PA PA (Particle agglutination) Particle aggregation reaction A method to determine the presence of antibody or antigen by performing antigen-antibody reaction using gelatin particles, etc. (sensitized particles) to which antigen or antibody is adsorbed (bound) and the presence or absence of aggregation due to antigen-antibody reaction.	Negative (less than 100) (x)
Anti-thyroid peroxidase antibody (anti-TPO antibody)（Suspended beyond orders placed 09-30-2021）	Serum 0.5	S09 ↓ A00			2-4	ECLIA ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.	Less than 16(IU/mL)
Anti-thyroid thyroglobulin antibody (Thyroid test)（Suspended beyond orders placed 12-01-2022）	Serum 0.3	S09 ↓ A00			2-4	PA PA (Particle agglutination) Particle aggregation reaction A method to determine the presence of antibody or antigen by performing antigen-antibody reaction using gelatin particles, etc. (sensitized particles) to which antigen or antibody is adsorbed (bound) and the presence or absence of aggregation due to antigen-antibody reaction.	Negative (less than 100) (x)
Immune complex (C1q)（Suspended beyond orders placed 05-14-2022）	Serum 0.3	S09 ↓ A00			2-4	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	3.0 or less (μg/mL)

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