TEST DIRECTORY

PCR (Polymerase chain reaction)
A method for exponentially amplifying the target DNA region by using the fact that DNA dissociates from double-stranded DNA to single-stranded DNA by heating and returns to double-stranded DNA by cooling, binding the target primer to single-stranded DNA as a template, and repeatedly performing DNA synthesis by the transcription reaction of DNA polymerase.
Sanger sequencing
This method uses DNA polymerase, a DNA replication enzyme, to synthesize DNA fragments whose ends correspond to specific bases, and currently uses dideoxynucleotides labeled with four different fluorescent dyes in the elongation reaction. Sequences are determined by using a capillary electrophoresis system to detect the type of fluorescent dye of dye-terminator and the length of DNA fragments, incorporated randomly in the elongation reaction from a single primer that binds specifically to the template DNA.

PCR (Polymerase chain reaction)
A method for exponentially amplifying the target DNA region by using the fact that DNA dissociates from double-stranded DNA to single-stranded DNA by heating and returns to double-stranded DNA by cooling, binding the target primer to single-stranded DNA as a template, and repeatedly performing DNA synthesis by the transcription reaction of DNA polymerase.
Sanger sequencing
This method uses DNA polymerase, a DNA replication enzyme, to synthesize DNA fragments whose ends correspond to specific bases, and currently uses dideoxynucleotides labeled with four different fluorescent dyes in the elongation reaction. Sequences are determined by using a capillary electrophoresis system to detect the type of fluorescent dye of dye-terminator and the length of DNA fragments, incorporated randomly in the elongation reaction from a single primer that binds specifically to the template DNA.

PCR (Polymerase chain reaction)
A method for exponentially amplifying the target DNA region by using the fact that DNA dissociates from double-stranded DNA to single-stranded DNA by heating and returns to double-stranded DNA by cooling, binding the target primer to single-stranded DNA as a template, and repeatedly performing DNA synthesis by the transcription reaction of DNA polymerase.
Sanger sequencing
This method uses DNA polymerase, a DNA replication enzyme, to synthesize DNA fragments whose ends correspond to specific bases, and currently uses dideoxynucleotides labeled with four different fluorescent dyes in the elongation reaction. Sequences are determined by using a capillary electrophoresis system to detect the type of fluorescent dye of dye-terminator and the length of DNA fragments, incorporated randomly in the elongation reaction from a single primer that binds specifically to the template DNA.

PCR (Polymerase chain reaction)
A method for exponentially amplifying the target DNA region by using the fact that DNA dissociates from double-stranded DNA to single-stranded DNA by heating and returns to double-stranded DNA by cooling, binding the target primer to single-stranded DNA as a template, and repeatedly performing DNA synthesis by the transcription reaction of DNA polymerase.
Sanger sequencing
This method uses DNA polymerase, a DNA replication enzyme, to synthesize DNA fragments whose ends correspond to specific bases, and currently uses dideoxynucleotides labeled with four different fluorescent dyes in the elongation reaction. Sequences are determined by using a capillary electrophoresis system to detect the type of fluorescent dye of dye-terminator and the length of DNA fragments, incorporated randomly in the elongation reaction from a single primer that binds specifically to the template DNA.

PCR (Polymerase chain reaction)
A method for exponentially amplifying the target DNA region by using the fact that DNA dissociates from double-stranded DNA to single-stranded DNA by heating and returns to double-stranded DNA by cooling, binding the target primer to single-stranded DNA as a template, and repeatedly performing DNA synthesis by the transcription reaction of DNA polymerase.
Sanger sequencing
This method uses DNA polymerase, a DNA replication enzyme, to synthesize DNA fragments whose ends correspond to specific bases, and currently uses dideoxynucleotides labeled with four different fluorescent dyes in the elongation reaction. Sequences are determined by using a capillary electrophoresis system to detect the type of fluorescent dye of dye-terminator and the length of DNA fragments, incorporated randomly in the elongation reaction from a single primer that binds specifically to the template DNA.

A method that uses a next-generation sequencer to simultaneously determine the base sequences of a huge number of DNA fragments.