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biochemical test
organic acids

Laboratory：Akiruno

○organic acids

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Lactic acid	Deproteination solution 0.4	M30 ↓ A00			2-4	Enzymatic method using lactic acid oxidase Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	3.0-17.0 (in whole blood) (mg/dL)
Pyruvic acid	Deproteination solution 0.4	M30 ↓ A00			2-4	Enzymatic method using pyruvate oxidase Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	0.30-0.94(whole blood) (mg/dL)
Urinary oxalic acid	acidic urine storage 3	A00		（21 days）	3-9	Capillary electrophoresis method Electrophoresis method A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.	M 10.3-41.5　F 9.0-37.7(mg/day)
Ketone bodies fractionation	Serum (venous blood) 0.5	S09 ↓ A00		（7 days）	2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	Acetoacetic acid 55 or less 3-Hydroxybutyric acid 85 or less Total ketone bodies 130 or less (μmol/L)
Ketone bodies fractionation	Serum (arterial blood) 0.5	S09 ↓ A00		（7 days）	2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	Ketone body ratio Acetoacetic acid/3-hydroxybutyric acid ratio 0.7 or more
Quantitative acetone	Serum 0.7	S7P ↓ A00		（15 days）	7-10	Gas-Chromatograph method Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.	Less than 5 (μg/mL)
Quantitative Urinary acetone	Partial urine 1.2	U00			7-10	Gas-Chromatograph method Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.	Less than 5 (μg/mL)
Organic acid-screening	Partial urine 5	U00			7-14	Gas chromatography method (GC-MS) Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.
Quantitative albumin（Suspended beyond orders placed 12-23-2022）	Partial urine 0.5	U00		（1 month）	6-12	Enzymatic method Enzyme method The measurement principle is the same as the colorimetric method, and the measurement substance is specifically measured using an enzyme.	138-1010(mg/L)
Quantitative albumin（Suspended beyond orders placed 12-23-2022）	Serum 1.5	S09 ↓ A00			6-12	Enzymatic method Enzyme method The measurement principle is the same as the colorimetric method, and the measurement substance is specifically measured using an enzyme.	1.3-2.6(mg/dL)