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  • tumor-associated antigen

Laboratory：Akiruno

○tumor-associated antigen

TEST NAME

 SPECIMEN
REQUIREMENT
（ｍL）		 CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY)		 TURNAROUND
TIME (DAY)		 METHODOLOGY REFERENCE RANGE
(UNIT)

INACTIVE
Sialyl Tn antigen (STN)（Temporarily suspended beyond orders placed 08-21-2024）

Serum
0.3
S09
↓
A00 		Refrigeration
 2-5 RIA solid phase method

IRMA (Immuno radio metric assay)
Immunoradiometric assay
A type of RIA in which an immobilized antibody is reacted with an antigen, and then an antibody labeled with a radioactive isotope (RI) is added to the antigen. A method of causing a secondary reaction.
It is also called the sandwich method because the immobilized antibody and labeled antibody bind with the antigen sandwiched between them.

 45 or less (U/mL)

Carcinoembryonic antigen (CEA)

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 5.0 or less (ng/mL)

α-fetoprotein quantitative(AFP)

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 10.0 or less (ng/mL)

Basic fetoprotein (BFP)

Immediately
Serum
0.3
S7P
↓
A00 		Refrigeration
 2-8 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 Less than 75 (ng/mL)

Human epididymis protein 4 (HE4)

Serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 CLIA

CLIA (Chemiluminescent immunoassay)
Chemiluminescent immunoassay method
After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.

 Premenopausal women: 70.0 or less
. Postmenopausal women: 140 or less
(pmol/L)

CA125

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 35.0 or less (U/mL)

CA15-3

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 25.0 or less (U/mL)

BCA225

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 160(U/mL)

CA19-9

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 37.0 or less (U/mL)

CA72-4

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 ECLIA

ECLIA (Electro chemiluminescence immunoassay)
Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.

 10.0 or less (U/mL)

CA54/61

Serum
0.5
S09
↓
A00 		Refrigeration
 4-17 ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 12 or less (U/mL)

DUPAN-2

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 150 or less (U/mL)

APOA2 isoform

Plasma
0.3
PN2,PN5
↓
A00 		Refrigeration
 5-7 ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 APOA2-i Index 59.5 or more
(μg/mL)

APOA2 isoform

Serum
0.3
S09
↓
A00 		Refrigeration
 5-7 ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 APOA2-i Index 59.5 or more
(μg/mL)

Sialyl Lex-i antigen (SLX)

Serum
0.2
S09
↓
A00 		Refrigeration
 2-4 RIA solid phase method (IRMA)

IRMA (Immuno radio metric assay)
Immunoradiometric assay
A type of RIA in which an immobilized antibody is reacted with an antigen, and then an antibody labeled with a radioactive isotope (RI) is added to the antigen. A method of causing a secondary reaction.
It is also called the sandwich method because the immobilized antibody and labeled antibody bind with the antigen sandwiched between them.

 38 or less (U/mL)

Sialyl Lexantigen (CSLEX)

Serum
0.4
S09
↓
A00 		Refrigeration
 2-8 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 Less than 8.0 (U/mL)

NCC-ST-439

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 Female
Under 49 years old
Less than 7.0
Over 50 years old
Less than 4.5
Male Less than 4.5 (U/mL)

SPan-1

Serum
0.2
S09
↓
A00 		Refrigeration
 2-4 RIA solid phase method (IRMA)

IRMA (Immuno radio metric assay)
Immunoradiometric assay
A type of RIA in which an immobilized antibody is reacted with an antigen, and then an antibody labeled with a radioactive isotope (RI) is added to the antigen. A method of causing a secondary reaction.
It is also called the sandwich method because the immobilized antibody and labeled antibody bind with the antigen sandwiched between them.

 30 or less (U/mL)

SCC

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 2.5 or less (ng/mL)

Prostate-specific antigen (PSA)

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 4.00 or less (ng/mL)

PSA F/T ratio

Serum
0.8
S09
↓
A00 		Freeze
（21 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 25 (Cutoff value at PSA 4.00-10.0ng/mL) (%)

γ-seminoprotein (γ-Sm)

Serum
0.5
S09
↓
A00 		Freeze
（21 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 4.0 or less (ng/mL)

CYFRA (Cytokeratin fragment 19)

Serum
0.8
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 3.5 or less (ng/mL)

5-S-Cysteinyl dopa (5-S-CD)

Serum
1.0
S09
↓
A00 		Freeze
 3-7 HPLC

HPLC (High performance liquid chromatography)
High performance liquid chromatography
A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.

 1.5-8.0(nmol/L)

Neuron specific enolase (NSE)

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 ECLIA

ECLIA (Electro chemiluminescence immunoassay)
Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.

 16.3 or less (ng/mL)

PIVKA-Ⅱ

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 40 (mAU/mL)

PIVKA-Ⅱ

Plasma
0.5
PN2,PN5
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 40 (mAU/mL)

Pro-gastrin-releasing peptide (ProGRP)

Plasma
0.4
PN2,PN5
↓
A00 		Freeze
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 81 (pg/mL)

Anti-p53 antibody

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 1.30 or less (U/mL)

Serum HER2 protein

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLIA

CLIA (Chemiluminescent immunoassay)
Chemiluminescent immunoassay method
After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.

 15.2 or less (ng/mL)

Soluble mesothelin related peptide

Serum
0.3
S09
↓
A00 		Refrigeration
（28 days）
 4-10 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 1.5 (nmol/L)

Specified days
CCR4 protein〔FCM〕

Blood (heparin added)
5.0
PH5 Room temperature
 3-5 Flow cytometry(FCM)

Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.

 Negative

INACTIVE
TPA（Suspended beyond orders placed 04-30-2024）

Serum
0.5
S09
↓
A00 		Freeze
 4-6 CLIA

CLIA (Chemiluminescent immunoassay)
A method in which after an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, in which the luminescence intensity of a chemiluminescent substrate is measured.

 Less than 75 (U/L)

INACTIVE
Prostate acid phosphatase (PAP)（Suspended beyond orders placed 12-01-2016）

Serum
0.5
S09
↓
A00 		Freeze
（21 days）
 2-4 DCAP-P substrate method 3.2 or less (U/L)

INACTIVE
PSA F/T Ratio Tandem（Suspended beyond orders placed 01-06-2017）

Serum
S09
↓
A00 		Freeze
 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
A method in which an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, a chemiluminescent substrate is added and the luminescence intensity is measured.

 25 (cutoff value in gray zone) (%)

INACTIVE
CA602（Suspended beyond orders placed 03-31-2021）

Serum
0.5
S09
↓
A00 		Refrigeration
 3-9 ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 63 or less (U/mL)

INACTIVE
PSA-ACT（Suspended beyond orders placed 03-31-2021）

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLIA

CLIA (Chemiluminescent immunoassay)
A method in which after an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, in which the luminescence intensity of a chemiluminescent substrate is measured.

 3.4 or less (ng/mL)

INACTIVE
Nuclear matrix protein 22, Urine (NMP22)（Suspended beyond orders placed 03-29-2024）

Immediately
Partial urine
5.0
U80 Refrigeration
（21 days）
 2-4 ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 Less than 12.0(U/mL)

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