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microbiological test
culture & identify test

Laboratory：Akiruno

○culture & identify test

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Virus isolation	See below	V10			4-69	Cytopathic effect, hemocyte adsorption phenomenon, red blood cell agglutination reaction
Virus identification	*See 1	V10			13-77	Neutralization reaction using standard antiserum, immunofluorescence antibody method, hemagglutination inhibition reaction
Mycoplasma Genitarium rRNA Qualitative（Suspended beyond orders placed 09-02-2021）	Secretion	V20			3-9	TMA TMA (Transcription mediated amplification) A method of amplifying RNA using two types of enzymes and two types of primers and substrates. Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.	Negative
Simultaneous identification of Neisseria gonorrhoeae and Chlamydia trachomatis rRNA（Suspended beyond orders placed 09-02-2021）	Secretion	V20			2-4	TMA TMA (Transcription mediated amplification) A method of amplifying RNA using two types of enzymes and two types of primers and substrates. Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.	Negative
Simultaneous identification of Neisseria gonorrhoeae and Chlamydia trachomatis rRNA（Suspended beyond orders placed 09-02-2021）	Partial urine 2.0	V20		（30 days）	2-4	TMA TMA (Transcription mediated amplification) A method of amplifying RNA using two types of enzymes and two types of primers and substrates. Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.	Negative
Simultaneous identification of Neisseria gonorrhoeae and Chlamydia trachomatis rRNA（Suspended beyond orders placed 09-02-2021）	Throat swab fluid	V20			2-4	TMA TMA (Transcription mediated amplification) A method of amplifying RNA using two types of enzymes and two types of primers and substrates. Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.	Negative
Simultaneous identification of Neisseria gonorrhoeae and Chlamydia trachomatis rRNA（Suspended beyond orders placed 09-02-2021）	Gargling liquid 2.0	V20		（30 days）	2-4	TMA TMA (Transcription mediated amplification) A method of amplifying RNA using two types of enzymes and two types of primers and substrates. Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.	Negative
Mycoplasma pneumoniae DNA Qualitative [QProbe]（Suspended beyond orders placed 11-14-2019）	Throat swab fluid	ARR		（28 days）	2-4	PCR(QProbe)	Negative(-)
Mycoplasma Genitarium rRNA Qualitative（Suspended beyond orders placed 09-02-2021）	Partial urine 2.0	V20			3-9	TMA TMA (Transcription mediated amplification) A method of amplifying RNA using two types of enzymes and two types of primers and substrates. Double-stranded DNA is synthesized from the extracted RNA using reverse transcriptase, and this double-stranded DNA is used as a template to synthesize RNA by RNA polymerase transcription reaction, which is repeated to amplify the desired RNA region.	Negative