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cellular immunity test
*Lymphocyte surface marker analysis by two-color analysis

Laboratory：Akiruno

○*Lymphocyte surface marker analysis by two-color analysis

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Two-colorCD4/CD45RA	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD4(-)45RA(+)30.0-57.0 CD4(-)45RA(-)5.0-23.0 CD4(+)45RA(-)15.0-34.0 CD4(+)45RA(+)9.0-37.0(%)
Two-colorCD4/CD29	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD4(-)CD29(+)22.0-59.0 CD4(-)CD29(-)12.0-38.0 CD4(+)CD29(-)9.0-34.0 CD4(+)CD29(+)11.0-26.0(%)
Two-colorCD11b/CD8	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD11b(-)CD8(+) 11.0-40.0 CD11b(-)CD8(-) 29.0-64.0 CD11b(+)CD8(-) 8.0-32.0 CD11b(+)CD8(+) 6.0-27.0 Bright 20.0 or less Dull 3.0-17.0(%)
Two-colorHLA-DR/CD4	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	HLA-DR(+)CD4(-)8.0-37.0 HLA-DR(-)CD4(-)24.0-61.0 HLA-DR(-)CD4(+)22.0-51.0 HLA-DR(+)CD4(+)1.0-12.0(%)
Two-colorHLA-DR/CD8	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	HLA-DR(-)CD8(+)15.0-49.0 HLA-DR(-)CD8(-)28.0-62.0 HLA-DR(+)CD8(-)6.0-25.0 HLA-DR(+)CD8(+)2.0-27.0(%)
Two-colorCD57/CD16	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD57(-)CD16(+)1.0-15.0 CD57(-)CD16(-)49.0-87.0 CD57(+)CD16(-)2.0-40.0 CD57(+)CD16(+)2.0-27.0(%)
Two-colorCD16/CD56	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD16(-)CD56(+) 2.0-20.0 CD16(-)CD56(-) 57.0-91.0 CD16(+)CD56(-) 9.0 or less CD16(+)CD56(+) 4.0 -33.0(%)
Two-colorCD45RO/CD4	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD45RO(-)CD4(+)6.0-34.0 CD45RO(-)CD4(-)28.0-58.0 CD45RO(+)CD4(-)7.0-32.0 CD45RO(+)CD4(+)10.0-32.0(%)
Two-colorCD4/CD8	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD4(-)CD8(+) 22.0-54.0 CD4(-)CD8(-) 14.0-38.0 CD4(+)CD8(-) 23.0-52.0 CD4(+)CD8(+) 7.0 or less (%) CD4/CD8 ratio 0.40-2.30
Two-colorHLA-DR/CD3	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	HLA-DR(+)CD3(-)3.0-18.0 HLA-DR(-)CD3(-)7.0-36.0 HLA-DR(-)CD3(+)33.0-75.0 HLA-DR(+)CD3(+)5.0-33.0(%)
Two-colorCD4/CD25	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD4(+)CD25(-)15.0-39.0 CD4(-)CD25(-)37.0-69.0 CD4(-)CD25(+)2.0-14.0 CD4(+)CD25(+)6.0-21.0(%)
Two-colorCD20/CD5	Blood (heparin added) 3.0	PH5			3-5	Two-color flow cytometry Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.	CD20(+)CD5(-) 9.0 or less CD20(-)CD5(-) 9.0-40.0 CD20(-)CD5(+) 54.0-87.0 CD20(+)CD5(+) 13.0 below(%)