Laboratory:Akiruno
- TOP
- gene-related test
- genetic tests for hematopoietic tumor
Laboratory:Akiruno
○genetic tests for hematopoietic tumor
TEST NAME |
SPECIMEN REQUIREMENT (mL) |
CONTAINER | CAP COLOR | STORE TEMPERATURE (STABILITY) |
TURNAROUND TIME (DAY) |
METHODOLOGY | REFERENCE RANGE (UNIT) |
---|---|---|---|---|---|---|---|
FLT3/ITD mutation analysis
|
bone marrow aspirate
1.0 |
H00 |
|
7-11 |
PCR PCR (Polymerase chain reaction) |
No ITD mutation detected | |
FLT3/ITD mutation analysis
|
Blood (EDTA-2Na added)
2.0 |
PN2,PN5 |
|
7-11 |
PCR PCR (Polymerase chain reaction) |
No ITD mutation detected | |
FLT3 mutation analysis ITD/TKD
|
bone marrow aspirate
1.0 |
H00 |
(7 days) |
4-8 | PCR-capillary electrophoresis method | Negative | |
FLT3 mutation analysis ITD/TKD
|
Blood (heparin added)
3.0 |
PH5 |
(7 days) |
4-8 | PCR-capillary electrophoresis method | Negative | |
NPM1 mutation analysis
|
bone marrow aspirate
1.0 |
H00 |
|
10-16 |
PCR (Real Time PCR) Real-time PCR |
No mutations detected | |
NPM1 mutation analysis
|
Blood (EDTA-2Na added)
2.0 |
PN2,PN5 |
|
10-16 |
PCR (Real Time PCR) Real-time PCR |
No mutations detected | |
KIT sequence analysis (leukemia)
|
bone marrow aspirate
1.0 |
H00 |
|
10-14 |
Direct sequence method Direct sequencing method |
No mutations detected | |
KIT sequence analysis (leukemia)
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
10-14 |
Direct sequence method Direct sequencing method |
No mutations detected | |
JAK2V617F gene mutation analysis
|
Blood (EDTA-2Na added)
5.0 |
PN5 |
(3 days) |
4-8 | Allele specific quantitative PCR (real time PCR) | 1.000% or less negative | |
MPN gene mutation analysis
|
Blood (EDTA-2Na added)
5.0 |
PN5 |
(3 days) |
4-8 |
PCR PCR (Polymerase chain reaction) |
No mutations detected | |
MYD88 gene mutation analysis
|
tissue
125mg (5×5×5mm) |
ARR |
|
12-14 |
Direct sequence method Direct sequencing method |
||
CD79B gene mutation analysis
|
tissue
125mg (5×5×5mm) |
ARR |
|
12-14 |
Direct sequence method Direct sequencing method |
||
EZH2 gene mutation analysis
|
Unstained specimen slide
5 sheets thickness 5μm |
Z10 |
|
7-11 |
PCR (Real Time PCR) Real-time PCR |
||
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
(copy/μgRNA) | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
(copy/μgRNA) | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
4-6 |
RT-PCR (Real Time PCR) Real-time PCR |
(copy/μgRNA) | |
|
bone marrow aspirate
1.0 |
H00 |
|
4-6 |
RT-PCR (Real Time PCR) Real-time PCR |
(copy/μgRNA) | |
|
bone marrow aspirate
1.0 |
H00 |
(1 day) |
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
(1 day) |
4-6 |
RT-PCR (Real Time PCR) Real-time PCR |
||
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
(1 day) |
4-6 |
RT-PCR (Real Time PCR) Real-time PCR |
||
|
bone marrow aspirate
1.0 |
H00 |
(1 day) |
4-6 |
RT-PCR (Real Time PCR) Real-time PCR |
||
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
(1 day) |
10-14 |
Direct sequence method Direct sequencing method |
||
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
(1 day) |
10-14 |
Direct sequence method Direct sequencing method |
||
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
(1 day) |
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
||
|
bone marrow aspirate
1.0 |
H00 |
(1 day) |
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
||
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
(1 day) |
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
(1 day) |
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
(1 day) |
10-14 |
Direct sequence method Direct sequencing method |
||
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
(1 day) |
10-14 |
Direct sequence method Direct sequencing method |
||
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
6-9 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No detection of chimeric mRNA (copy/μgRNA) |
|
|
bone marrow aspirate
1.0 |
H00 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
7.0 |
PN7 |
|
7-11 |
RT-PCR RT-PCR (Reverse transcriptase-polymerase chain reaction) |
No detection of chimeric mRNA | |
|
Blood (EDTA-2Na added)
5.0 |
PN5 |
|
8-12 |
PCR (Real Time PCR) Real-time PCR |
No mutations detected | |
|
bone marrow aspirate
1.0 |
H00 |
|
8-12 |
PCR (Real Time PCR) Real-time PCR |
No mutations detected | |
|
bone marrow aspirate
1.0 |
H00 |
(1 day) |
3-5 |
RT-PCR (Real Time PCR) Real-time PCR |
No chimeric mRNA detected (copy/μgRNA) |
|
|
Blood (EDTA-2Na added)
5.0 |
PN5 |
|
4-8 |
PCR PCR (Polymerase chain reaction) |
No mutations detected |