TEST DIRECTORY

IFA (Indirect Fluorescent antibody method)
Indirect fluorescent antibody method
A method in which an antigen-antibody reaction is performed against the target antigen using an antibody labeled with a fluorescent dye, and the fluorescence intensity is measured under a fluorescence microscope. . There is a direct method in which an antibody labeled with a fluorescent dye is reacted directly, and an indirect method in which the antibody is reacted with an antigen and then a secondary reaction is performed with an antibody labeled with a fluorescent dye.

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.