TEST DIRECTORY

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

HPLC (High performance liquid chromatography)
High performance liquid chromatography
A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.

RIA (Radio immunoassay)
Radioimmunoassay
A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity.
Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

Flow cytometry
A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed.
When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

ECLIA (Electro chemiluminescence immunoassay)
Electrochemiluminescence immunoassay
A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

Flow cytometry
A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed.
When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.

Flow cytometry
A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed.
When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

Flow cytometry
A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed.
When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.

Flow cytometry
A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed.
When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.

MALDI-TOF-MS
A method that combines laser irradiation of a mixture of a laser light-absorbing matrix and analyte to ionize the protein without degrading it (MALDI) and mass analysis using the fact that the time-of-flight of ions varies depending on the mass-to-charge ratio of the generated ions (TOF-MS).
MALDI: Matrix-Assisted Laser Desorption/lonization
TOF-MS: Time-of-Flight Mass Spectrometry