TEST DIRECTORY

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Laboratory：Akiruno

○cytokines

TEST NAME

 SPECIMEN
REQUIREMENT
（ｍL）		 CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY)		 TURNAROUND
TIME (DAY)		 METHODOLOGY REFERENCE RANGE
(UNIT)

Soluble IL-2 receptor (sIL-2R)

Serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 157-474(U/mL)

TARC (Th2 chemokine)

Serum
0.3
S09
↓
A00 		Refrigeration
（28 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 6 months to 12 months old: less than 1367
1 year to 2 years old: less than 998
2 years to 16 years old: less than 743
16 years and older: less than 450
(pg/mL)

TARC (drug-induced hypersensitivity syndrome)

Serum
0.3
S09
↓
A00 		Refrigeration
（28 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 4000 (cutoff value)(pg/mL)

TARC (COVID-19)

Immediately
Serum
0.3
S09
↓
A00 		Refrigeration
（28 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Cutoff value 95.1 or higher
(pg/mL)

Interferon-lambda3 (IFN-λ3)

Serum
0.5
S09
↓
A00 		Refrigeration
（22 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Cutoff value less than 13.6 (pg/mL)

Human interferon γ〔EIA〕

Serum
0.5
S09
↓
A00 		Freeze
（1 month）
 Please contact us in advance. Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 0.1 or less (reference value) (IU/mL)

IL-1β (interleukin-1β)

Serum
0.5
S09
↓
A00 		Freeze
（1 month）
 Please contact us in advance. ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 10 or less (reference value) (pg/mL)

IL-4 (interleukin-4) (high sensitivity)

Serum
0.3
S09
↓
A00 		Freeze
（1 month）
 Please contact us in advance. CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 6.0 or less (Reference value)(pg/mL)

Interleukin-6 (IL-6)

Immediately
Serum
0.5
S09
↓
A00 		Freeze
（21 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 5.8 or less (pg/mL)

IL-6 (interleukin-6)

Serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-5 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 4.0 or less (pg/mL)

IL-8 (interleukin-8)

Culture medium
0.5
A00 Freeze
 Please contact us in advance. ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 (pg/mL)

IL-8 (interleukin-8)

Plasma
0.5
PN2,PN5
↓
A00 		Freeze
 Please contact us in advance. ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 2.0 or less (Reference value)(pg/mL)

IL-10 (interleukin-10)

Serum
0.5
S09
↓
A00 		Freeze
（1 month）
 Please contact us in advance. ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 5 or less (Reference value)(pg/mL)

INACTIVE
Soluble IL-2 Receptor（Suspended beyond orders placed 03-29-2019）

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
A method in which an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, a chemiluminescent substrate is added and the luminescence intensity is measured.

 145-519(U/mL)

INACTIVE
IL-5 (Interleukin-5)（Suspended beyond orders placed 03-29-2019）

Serum
0.5
S09
↓
A00 		Freeze
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 3.9 or less (Reference value)(pg/mL)

INACTIVE
TGF-β1（Suspended beyond orders placed 12-03-2020）

Immediately Refrigerated
Plasma
0.5
PN2,PN5
↓
A00 		Freeze
（1 month）
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 1.56-3.24 (reference value) (ng/mL)

INACTIVE
G-CSF (granulocyte colony-stimulating factor)（Suspended beyond orders placed 12-03-2020）

Serum
0.5
S09
↓
A00 		Freeze
（1 month）
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 39.0 or less (Reference value)(pg/mL)

INACTIVE
GM-CSF (Granulocyte/Macrophage Colony Stimulating Factor)（Suspended beyond orders placed 03-29-2019）

Culture medium
0.5
A00 Freeze
（1 month）
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 (pg/mL)

INACTIVE
Soluble VCAM-1（Suspended beyond orders placed 03-29-2019）

Serum
0.3
S09
↓
A00 		Freeze
（1 month）
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 277-836(Reference value)(ng/mL)

INACTIVE
Soluble ICAM-1（Suspended beyond orders placed 03-29-2019）

Serum
0.3
S09
↓
A00 		Freeze
（1 month）
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 98.8-320(Reference value)(ng/mL)

INACTIVE
Soluble ELAM-1（Suspended beyond orders placed 03-29-2019）

Serum
0.3
S09
↓
A00 		Freeze
（1 month）
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 17.9-79.2 (reference value) (ng/mL)

INACTIVE
IL-1RA (Interleukin-1 Receptor Antagonist)（Suspended beyond orders placed 11-30-2017）

Plasma
0.5
PN2,PN5
↓
A00 		Freeze
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 105-1062 (reference value) (pg/mL)

INACTIVE
IL-1α (interleukin-1α)（Suspended beyond orders placed 11-30-2017）

Serum
1.0
S09
↓
A00 		Freeze
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 (pg/mL)

INACTIVE
IL-1α (interleukin-1α)（Suspended beyond orders placed 11-30-2017）

Culture medium
1.0
A00 Freeze
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 (pg/mL)

INACTIVE
IL-3 (Interleukin-3)（Suspended beyond orders placed 11-30-2017）

Culture medium
1.0
A00 Freeze
（1 month）
 Please confirm in advance ELISA

ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.

 (pg/mL)

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