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endocrinology test
pancreatic hormones and gut hormones

Laboratory：Akiruno

○pancreatic hormones and gut hormones

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Insulin	Serum 0.4	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Before loading 1.84-12.2(μIU/mL)
C-ペプチド(CPR)	Serum 0.4	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Before loading 0.61-2.09(ng/mL)
C-ペプチド(CPR)	Urine storage 1	U00		（20 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	29.2-167(μg/day)
Pancreatic glucagon	Plasma 0.5	PAP ↓ A00		（28 days）	5-11	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Fasting 5.4 to 55.0 (pg/mL)
Gastrin	Serum 0.5	S09 ↓ A00		（21 days）	2-8	RIA PEG method RIA (Radio immunoassay) Radioimmunoassay A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity. Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	11.9-46.9 (pmol/L)
Glucagon (IRG)（Suspended beyond orders placed 03-31-2021）	Plasma 0.5	PAP ↓ A00		（21 days）	4-10	RIA2 antibody method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	70-174(pg/mL)
Gastrin（Suspended beyond orders placed 03-31-2022）	Serum 0.4	S09 ↓ A00			2-5	RIA PEG method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	Before loading 200以下(pg/mL)