TEST DIRECTORY

AND OR

TOP
biochemical test
vitamin and related materials

Laboratory：Akiruno

○vitamin and related materials

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
β-carotene	Serum 0.6	S09 ↓ ASS		（1 month）	5-11	HPLC HPLC (High performance liquid chromatography) High performance liquid chromatography A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.	M 6.6-47.6 F 20.4-105.2 (μg/dL)
Vitamin A	Serum 0.4	S09 ↓ ASS			4-6	HPLC HPLC (High performance liquid chromatography) High performance liquid chromatography A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.	97-316 (IU/dL)
Vitamin B₁	Blood (EDTA-2K added) 0.5	PKF		（6 months）	3-5	LC/MS/MS LC/MS/MS (Liquid chromatography tandem mass spectrometry) Liquid chromatography tandem quadrupole mass spectrometry Liquid chromatography uses differences in affinity to decompose components of a target substance. , a method in which ions of a specific mass are further separated by mass using a mass spectrometer, dissociated and fragmented, and then these ions are detected.	24-66(ng/mL)
Vitamin B₂	Blood (EDTA-2K added) 0.5	PKF ↓ ASS		（1 month）	3-5	HPLC HPLC (High performance liquid chromatography) High performance liquid chromatography A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.	66.1-111.4 (ng/mL)
Vitamin B₆	Serum 0.5	S09 ↓ ASS			3-5	HPLC HPLC (High performance liquid chromatography) High performance liquid chromatography A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.	Pyridoxamine M 0.6 or less F 0.6 or less Pyridoxal M 6.0-40.0 F 4.0-19.0 Pyridoxine M 3.0 or less F 3.0 or less (ng/mL)
Vitamin B₁₂	Serum 0.6	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	180-914 (pg/mL)
Carnitine fractionation	Serum 0.5	S09 ↓ A00			2-4	enzyme cycling method	Total carnitine 45-91 Free carnitine 36-74 Acylcarnitine 6-23 (μmol/L)
Vitamin C (ascorbic acid)	(In serum) Deproteinized supernatant 0.5	M50 ↓ ASS		（7 days）	3-5	HPLC HPLC (High performance liquid chromatography) High performance liquid chromatography A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.	5.5-16.8 (in serum) (μg/mL)
25OH vitamin D(osteoporosis)	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Vitamin D deficiency: Less than 20.0 Vitamin D Insufficiency: 20.0-29.9 (ng/mL)
25-hydroxyvitamin D (Rickets/osteomalacia)	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Vitamin D deficiency 20 or less (ng/mL)
1,25-(OH)₂ Vitamin D	Serum 0.6	S09 ↓ A00			3-7	RIA2 antibody method RIA (Radio immunoassay) Radioimmunoassay A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity. Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	Adults 20.0-60.0 Children 20.0-70.0 (pg/mL)
Vitamin E	Serum 0.5	S09 ↓ ASS			7-13	Fluorescence method	0.75-1.41(mg/dL)
Vitamin E fractionation	Serum 0.5	S09 ↓ ASS			Please contact us in advance.	HPLC HPLC (High performance liquid chromatography) High performance liquid chromatography A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.	α-tocopherol 0.49-1.09 β-tocopherol 0.02 or less γ-tocopherol 0.05-0.17 δ-tocopherol 0.01 or less (mg/dL)
Vitamin K fractionation	Plasma 2.0	PC5 ↓ ASS		（17 days）	3-9	HPLC HPLC (High performance liquid chromatography) High performance liquid chromatography A method of liquid chromatography that uses a liquid as a mobile phase to achieve high speed and high precision separation using a densely packed column and a high pressure pump.	Vitamin K1 0.15-1.25 Vitamin K2 (MK-4) 0.10 or less (ng/mL)
Folic acid	Serum 0.6	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	4.0 or more (ng/mL)
Nicotinic acid (niacin)	Blood (heparin added) 1.5	PH5			7-13	Bioassay	4.7-7.9 (μg/mL)
25-OH vitamin D（Suspended beyond orders placed 06-30-2016）	Serum 0.2	S09 ↓ A00			5-11	RIA2 antibody method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	7-41(ng/mL)
25OH Vitamin D 〔RIA〕（Suspended beyond orders placed 12-03-2020）	Serum 0.2	S09 ↓ A00			5-11	RIA solid phase method IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.	(ng/mL)