TEST DIRECTORY

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Laboratory：Akiruno

○enzymes and related materials

TEST NAME

 SPECIMEN
REQUIREMENT
（ｍL）		 CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY)		 TURNAROUND
TIME (DAY)		 METHODOLOGY REFERENCE RANGE
(UNIT)

CK(CPK)

Serum
0.5
S09
↓
A00 		Refrigeration
（10 days）
 2-4 JSCC Standardization Compatibility Method M 62-287
F 45-163(U/L)

CK-MB(CPK-MB)

Serum
0.5
S09
↓
A00 		Refrigeration
（6 days）
 2-4 Immunoblocking - UV method

UV method (Ultraviolet absorption spectrophotometry)
Ultraviolet absorption spectrophotometry
The measurement principle is the same as the colorimetric method, and it is a method of measuring using ultraviolet wavelengths. Typically, near-ultraviolet wavelengths of 200 to 400 nm are used.

 MB 25 or less
TOTAL M 62-287
F 45-163
(U/L)

CK-MB(CPK-MB)

Serum
0.5
S09
↓
A00 		Freeze
 2-4 CLIA

CLIA (Chemiluminescent immunoassay)
Chemiluminescent immunoassay method
After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.

 5.0 or less (ng/mL)

CK (CPK)isoenzymes

Immediately
Serum
0.3
S09
↓
A00 		Freeze
 2-4 Agarose gel electrophoresis

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

 BB 2 or less MB 6 or less MM 93-99 (%)

Emergency
AST(GOT)

Serum
0.5
S09
↓
A00 		Refrigeration
（14 days）
 2-4 JSCC Standardization Compatibility Method 10-40(U/L)

Emergency
ALT(GPT)

Serum
0.5
S09
↓
A00 		Refrigeration
（14 days）
 2-4 JSCC Standardization Compatibility Method 5-40(U/L)

Emergency
LD(LDH)IFCC

Serum
0.5
S09
↓
A00 		Refrigeration
（3 days）
 2-4 IFCC Standardization Compliance Act 124-222(U/L)

LD(LDH) Isoenzyme

Serum
0.3
S09
↓
A00 		Room temperature
 2-4 Agarose gel electrophoresis

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

 LDH1 21-31 LDH2 28-35 LDH3 21-26 LDH4 7-14 LDH5 5-13 (%)

Alkaline phosphatase (ALP), IFCC

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 IFCC Standardization Compliance Act 38-113(U/L)

Bone alkaline phosphatase (BAP)

Serum
0.8
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 M3.7-20.9
Premenopausal women 2.9-14.5
Postmenopausal women 3.8-22.6
(μg/L)

Placental alkaline phosphatase (PLAP)

Cerebrospinal fluid
0.5
A00 Refrigeration
（28 days）
 2-6 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 (pg/mL)

Alkaline phosphatase isoenzymes(IFCC)

Serum
0.3
S09
↓
A00 		Refrigeration
（21 days）
 2-4 Agarose gel electrophoresis

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

 ALP10.0-5.3
ALP236.6-69.2
ALP325.2-54.2
ALP50.0-18.1(%)

γ-GT(γ-GTP)

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 JSCC Standardization Compatibility Method M 70 or less
F 30 or less
(U/L)

Aldolase

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 UV Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 2.1-6.1(U/L)

Cholinesterase (ChE)

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 JSCC Standardization Compatibility Method M 242-495
F 200-459
(U/L)

Guanase

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 1.0 or less (U/L)

Adenosine deaminase (ADA)

Serum
0.6
S09
↓
A00 		Refrigeration
 2-4 Enzymatic method

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 5.0-20.0(U/L)

Leucine aminopeptidase (LAP)

Serum
0.5
S09
↓
A00 		Refrigeration
（1 month）
 2-4 Enzymaticmethod(L-leucinep-nitroanilide)

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 35-73(U/L)

Emergency
Amylase (AMY)

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 JSCC Standardization Compatibility Method 37-125(U/L)

Amylase (AMY)

Partial urine
0.5
U00 Refrigeration
 2-4 Enzymatic method(Gal-G2-CNP substrate)

Enzymatic method
This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.

 65-700(U/L)

Amylase isoenzymes

Serum
0.3
S09
↓
A00 		Refrigeration
 2-4 Agarose gel electrophoresis

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

 TOTAL-S 36.0-84.3
TOTAL-P 15.7-64.0(%)

Amylase isoenzymes

Partial urine
0.5
U00 Refrigeration
 2-4 Agarose gel electrophoresis

Electrophoresis method
A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.

 TOTAL-S 17.7-61.3
TOTAL-P 38.7-82.3(%)

Amylase, type P, quantitative

Serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 JSCC Standardization Compatibility Method 21-64(U/L)

Lipase

Serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 Synthetic substrate colorimetric method 13-55(U/L)

Trypsin

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination turbidimetry

LA (Latex agglutination immunoassay)
Latex agglutination turbidimetry
An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.

 210-570(ng/mL)

Pancreatic phospholipase A2 (pancreatic PLA2)

Refrigerated
Serum
0.3
S09
↓
A00 		Freeze
（21 days）
 2-5 RIA solid phase method

IRMA (Immuno radio metric assay)
Immunoradiometric assay
A type of RIA in which an immobilized antibody is reacted with an antigen, and then an antibody labeled with a radioactive isotope (RI) is added to the antigen. A method of causing a secondary reaction.
It is also called the sandwich method because the immobilized antibody and labeled antibody bind with the antigen sandwiched between them.

 130-400(ng/dL)

Pancreatic phospholipase A2 (pancreatic PLA2)

Refrigerated
Plasma
0.3
PN2,PN5
↓
A00 		Freeze
 2-5 RIA solid phase method

IRMA (Immuno radio metric assay)
Immunoradiometric assay
A type of RIA in which an immobilized antibody is reacted with an antigen, and then an antibody labeled with a radioactive isotope (RI) is added to the antigen. A method of causing a secondary reaction.
It is also called the sandwich method because the immobilized antibody and labeled antibody bind with the antigen sandwiched between them.

 130-400(ng/dL)

Elastase 1

Serum
0.5
S09
↓
A00 		Freeze
 2-4 Latex immunoturbidimetry 300 or less (ng/dL)

Granulocyte elastase in cervical mucus

Cervical mucus
VP6 Freeze
（1 month）
 2-4 Latex agglutination immunoassay 1.60 or less (μg/mL)

Tartrate resistant acid phosphatase (TRACP-5b)

Serum
0.5
S09
↓
A00 		Freeze
 2-4 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 M 170-590
F(YAM) 120-420
(mU/dL)

Lysozyme

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry 5.0-10.2(μg/mL)

Lysozyme

Partial urine
0.4
U00 Refrigeration
 2-4 Nephelometry Less than 0.1 (μg/mL)

Angiotensin I Converting enzyme (ACE)

Serum
0.7
S09
↓
A00 		Refrigeration
 2-4 Kasahara method (Colorimetry) 8.3-21.4(U/L)

N-acetyl glucosaminidase (NAG)

Partial urine
0.5
U00 Refrigeration
 2-4 Colorimetry

Colorimetric method
A method in which the substance to be measured is converted into a colored substance, then irradiated with visible wavelengths, the absorbance is measured, and the color tone is compared with a standard solution.

 0.7-11.2(IU/L)

Pepsinogen

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 See below

Matrix metalloproteinase 3 (MMP-3)

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 LTIA M 36.9-121
F 17.3-59.7
(ng/mL)

Gastric cancer risk stratification (ABC classification)〔LA〕

Serum
1.0
S09
↓
A00 		Refrigeration
（21 days）
 2-4 Latex agglutination turbidimetry
CLEIA 		See below

Gastric cancer risk stratification (ABC classification), eradicated〔LA〕

Serum
1.0
S09
↓
A00 		Refrigeration
（21 days）
 2-4 Latex agglutination turbidimetry
CLEIA 		See below

INACTIVE
Gastric cancer risk screening (ABC classification)（Suspended beyond orders placed 03-31-2020）

Serum
0.8
S09
↓
A00 		Refrigeration
 2-4 EIA
CLEIA 		See below

INACTIVE
Acid phosphatase(AcP)（Suspended beyond orders placed 12-01-2016）

Serum
0.5
S09
↓
A00 		Freeze
（21 days）
 2-4 DCAP-P substrate method 14.4 or less (U/L)

INACTIVE
Gastric cancer risk screening (ABC classification), eradicated（Suspended beyond orders placed 03-31-2020）

Serum
0.8
S09
↓
A00 		Refrigeration
 2-4 EIA
CLEIA

INACTIVE
Mitochondria-AST(m-AST)(m-GOT)（Suspended beyond orders placed 03-31-2023）

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Protease method 7 or less (U/L)

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