TEST DIRECTORY

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Laboratory：Akiruno

○BMT (bone marrow transplant) Donor Male

CODE：0Y767 6

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Specified days
    BMT (bone marrow transplant) Donor Male
    Blood (heparin added)
    5.0
    PH5 Refrigeration
     8-14 G-band

    (Various) Band
    Culture lymphocytes or bone marrow cells, and fix metaphase cells. This method then stains the chromosomes with a dye to create a striped pattern (band), and analyzes the distribution and density of the band.
    G-Banding, which performs Giemsa staining after treatment with trypsin solution, C-Banding, which performs Giemsa staining after treatment with HCI, Ba (OH) 2, 2×SCC, these include Q-Banding, which involves staining with quinacrine mustard and then observing with a fluorescence microscope, and high-precision banding, which uses mitotic images from the end of prophase to the beginning of metaphase, and observes a larger number of bands than usual.
other material

COMMENT


Avoid freezing.
The available days are Monday through Friday (for both blood and bone marrow fluid.)
Please make your request with the name of the relevant disease.
Please see below for the names of diseases covered.
If it takes time to make a determination, the time required will be around 20 days.
About Submission of Chromosome Test The specimen must be submitted on the same day after collection.
Notes on Blood Disease Chromosome Test (G-Banding)
1. Gender information is required for G-banding determination, so please be sure to indicate your gender on the request form.
2. Ten million nucleated cells (1 x 107 cells) are required for bone marrow chromosome test. Collect bone marrow fluid aseptically to ensure that this quantity is sufficiently satisfied (This corresponds to 0.1 mL of bone marrow fluid when the number of nucleated cells in the bone marrow is 100,000 cells/μL, or 1 mL of bone marrow fluid when the number is 10,000 cells/μL.)
3.Chromosome mitotic images may not be obtained and thus test may not be possible during administration of steroids, alkylating agents, and antimetabolites.
4.When requesting with peripheral blood, test may not be possible if blast cells have not appeared in the blood, or if the blood from a patient in remission does not show chromosome mitotic figures.
Collect blood in the container shown below, mix well, and preserve refrigerated.
The specimen must be submitted on the same day after collection.

CONTAINER

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