Laboratory：Akiruno
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- chromosomal test
- *chromosomal tests (G-banding) Myeloid-related
- BMT (bone marrow transplant) Donor Male
Laboratory：Akiruno
○BMT (bone marrow transplant) Donor Male
CODE：0Y767 6
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
BMT (bone marrow transplant) Donor Male
Blood (heparin added)
5.0
PH5
8-14 G-band(Various) band
Culture lymphocytes or bone marrow cells, and fix metaphase cells. This method then involves staining the chromosomes with a dye to create striped patterns (bands), and analyzing the distribution and density of the bands.
G-Banding, Giemsa staining after treatment with trypsin solution; C-Banding, Giemsa staining after treatment with HCI, Ba(OH)2, 2×SCC; Q, staining with quinacrine mustard and observation with a fluorescence microscope. -Banding, a high-precision differential staining method that uses mitotic images from the end of prophase to the beginning of metaphase to increase the number of bands than usual.
TEST NAME | SPECIMEN | CONTAINER |
|---|---|---|
|
Blood (heparin added)
|
CAP COLOR | STORE | TURNAROUND |
|---|---|---|
|
| 8-14 |
METHODOLOGY | REFERENCE RANGE |
|---|---|
G-band (Various) band |
|
other material
COMMENT
Avoid freezing.
The days when we can accept orders (for both blood and bone marrow fluid) are from Monday to Friday.
Please make a request using the name of the disease.
Please see below for target disease names.
If the judgment takes time, the required number of days will be around 20 days.
About submitting a chromosome test Please submit the specimen on the same day after it is collected.
Notes on Blood Disease Chromosome Testing (G-Banding)
1. Sex information is required to determine G-banding, so please be sure to write your sex on the request form.
2. 10 million nucleated cells (1×107 cells) are required for bone marrow chromosome testing. Aseptically collect bone marrow fluid to fill this volume. (This corresponds to 0.1 mL of bone marrow fluid when the number of nucleated cells in the bone marrow is 100,000 cells/μL, and 1 mL of bone marrow fluid when the number of nucleated cells in the bone marrow is 10,000 cells/μL.)
3. Steroid drugs, alkylation During administration of drugs or antimetabolites, chromosomal mitotic figures may not be obtained and testing may not be possible.
4. When requesting peripheral blood, if there are no immature cells (blast) in the blood, or if the blood of a patient in remission cannot be tested because chromosomal mitotic figures cannot be obtained. .
Collect blood into the container shown below, mix well, and store in the refrigerator.
After collecting the specimen, please submit it on the same day.
CONTAINER
PH5 Previous container symbol G
With heparin (5mL vacuum blood sampling tube)
Contents: Heparin sodium 65IU
Storage method: Room temperature
Expiration period: 2 years from production
supplementary information
Hematological disorders detected by the chromosomal tests (G-banding)
- Please order a test for the name of disease concerned.
-
Myeloid-related CODE Disease 00886 8Myelodysplastic syndrome (MDS) 00895 8Myeloproliferative neoplasms (MPN) 00896 7Acute myelogenous leukemia (AML) 00919 1Bone marrow transplantation (BMT) 00920 9Others -
Lymphoid-related CODE Disease 00931 7Acute lymphocytic leukemia (ALL) 00933 5Chronic lymphocytic leukemia (CLL) 00949 8Malignant lymphoma (ML) 00951 5Multiple myeloma/plasma cell leukemia (MM/PL) 00958 8Others
