Laboratory:Akiruno
- TOP
- chromosomal test
- chromosomal test for solid tumors
- MYCN 2p24 Amplification
Laboratory:Akiruno
○MYCN 2p24 Amplification
CODE:06807 9
-
TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
MYCN 2p24 Amplification
tissue
5×5×5mm
H00
7-10 FISH(Various) Band
Culture lymphocytes or bone marrow cells, and fix metaphase cells. This method then stains the chromosomes with a dye to create a striped pattern (band), and analyzes the distribution and density of the band.
G-Banding, which performs Giemsa staining after treatment with trypsin solution, C-Banding, which performs Giemsa staining after treatment with HCI, Ba (OH) 2, 2×SCC, these include Q-Banding, which involves staining with quinacrine mustard and then observing with a fluorescence microscope, and high-precision banding, which uses mitotic images from the end of prophase to the beginning of metaphase, and observes a larger number of bands than usual.
COMMENT
Avoid freezing. Acceptable days are Monday through Friday. The main target disease is neuroblastoma.
About Submission of Chromosome Testing Submit the specimen on the same day after collection.
Float 5 x 5 x 5 mm of tissue in the designated container and preserve refrigerated.
Submit the specimen on the same day after collection.
CONTAINER
H00 旧容器記号 H
保存液入り (容器容量5mL)
内容:RPMI-1640 FBS 硫酸カナマイシン ノボヘパリンNa 炭酸水素Na HEPES
貯蔵方法:凍結
有効期間:色が薄いピンクの状態で使用してください。
(凍結時は淡黄色ですが解凍すると薄いピンク色に戻ります。)
supplementary information
染色体検査と関連する疾患
検査項目 | 主な対象疾患 | 主な用途 |
---|---|---|
del (1) 短腕欠失 | 脳腫瘍 | 治療感受性 |
神経芽細胞腫 | 予後推定 | |
MYCN 2p24増幅 | 神経芽細胞腫 | 予後推定 |
del (19) 長腕欠失 | 脳腫瘍 | 治療感受性 |
EWSR1 22q12転座 | ユーイング肉腫 P-NET |