Laboratory：Akiruno
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- gene-related test
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- Chimerism analysis after transplantation〔PCR〕
Laboratory：Akiruno
○Chimerism analysis after transplantation〔PCR〕
CODE：06151 7
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Chimerism analysis after transplantation〔PCR〕
Blood (EDTA-2Na added)
7.0
PN7
4-6 PCRPCR (Polymerase chain reaction)
Uses the fact that DNA dissociates from double strands to single strands when heated and returns to double strands when cooled, and binds the desired primer using single stranded DNA as a template. A method in which the target DNA region is exponentially amplified by repeatedly performing DNA synthesis using the transcription reaction of DNA polymerase.
other material
COMMENT
Avoid freezing. Acceptable days are Monday to Friday. In the case of recipients, the number of cells may decrease due to chemotherapy, etc., and DNA may not be recovered, so please submit a minimum number of cells of 2 x 105. Before transplantation, please conduct a "chimerism analysis pre-transplant recipient/donor [PCR]" test. This testing method increases the effects of contamination, so please be careful when handling the specimen.
Regarding requests for chimerism analysis testing
1.Chimerism analysis Post-transplant determination requires pre-transplant results. This is the acceptance condition after chimerism analysis transplantation.
2. Chimerism analysis If you are requesting a pre-transplant test, please be sure to indicate whether you have a history of transplantation on the request form.
Collect blood into the container shown below, mix well, and store in the refrigerator.
This testing method increases the influence of contamination, so please be careful when handling the specimen.
CONTAINER
PN7 旧容器記号 A 4
EDTA-2Na入り (真空採血量7mL)
内容：EDTA-2Na 10.5mg
貯蔵方法：室温
有効期間：製造から2年
