Chimerism analysis pre-transplant recipient 〔PCR〕

TOP
gene-related test
genetic tests related to transplantation
Chimerism analysis pre-transplant recipient 〔PCR〕

Laboratory：Akiruno

○Chimerism analysis pre-transplant recipient 〔PCR〕

CODE：06149 0

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Chimerism analysis pre-transplant recipient 〔PCR〕
Blood (EDTA-2Na added)
7.0
PN7
4-6 PCR
PCR (Polymerase chain reaction)
Uses the fact that DNA dissociates from double strands to single strands when heated and returns to double strands when cooled, and binds the desired primer using single stranded DNA as a template. A method in which the target DNA region is exponentially amplified by repeatedly performing DNA synthesis using the transcription reaction of DNA polymerase.

COMMENT

Avoid freezing. Acceptable days are Monday to Friday. In the case of recipients, the number of cells may decrease due to chemotherapy, etc., and DNA may not be recovered, so please submit a minimum number of cells of 2 x 10⁵. Please be sure to submit as a pair of recipient and supplier. This testing method increases the effects of contamination, so please be careful when handling the specimen.
Regarding requests for chimerism analysis testing
1.Chimerism analysis Post-transplant determination requires pre-transplant results. This is the acceptance condition after chimerism analysis transplantation. 　
2. Chimerism analysis If you are requesting a pre-transplant test, please be sure to indicate whether you have a history of transplantation on the request form.
Collect blood into the container shown below, mix well, and store in the refrigerator. Please be sure to submit as a pair of recipient and supplier.
This testing method increases the influence of contamination, so please be careful when handling the specimen.

CONTAINER

PN7 旧容器記号 A 4

EDTA-2Na入り (真空採血量7mL)

内容：EDTA-2Na　10.5mg
貯蔵方法：室温
有効期間：製造から2年

TEST DIRECTORY