MALT1 18q21 translocation | TEST DIRECTORY

TOP
chromosomal test
chromosomal test for leukemia/malignant lymphomas
MALT1 18q21 translocation

Laboratory：Akiruno

○MALT1 18q21 translocation

CODE：06141 8

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
MALT1 18q21 translocation
bone marrow aspirate
1.0
H00
7-10 FISH
FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
MALT1 18q21 translocation	bone marrow aspirate 1.0	H00

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
		7-10

METHODOLOGY	REFERENCE RANGE (UNIT)
FISH FISH (Fluorescence in situ hybridization) Fluorescence in situ hybridization Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope. There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

METHODOLOGY

REFERENCE RANGE
(UNIT)

FISH

FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

COMMENT

Avoid freezing. Acceptable days are Monday to Friday. In the case of gastric biopsy materials, please submit 5 or more in one H00 container.
About submitting a chromosome test: Please submit the specimen on the same day after it is collected.
Collect 1.0mL of bone marrow fluid aseptically into the container shown below, mix well, and store refrigerated. Please submit the specimen on the same day after collection.

CONTAINER

H00 Previous container symbol H

With the preservation solution (Container capability of 5 mL)

Contents: RPMI-1640　FBS　Kanamycin sulfate　Novo-heparin sodium　Sodium bicarbonate　HEPES
Storage method: Freeze
Expiration period: Use this after confirming that the solution color has turned to pale pink.
The color turns to pale yellow after freezing and to pale pink after thawing.

supplementary information

染色体検査で用いられる代表的な記号と略語、核型記載、増殖不良報告について

TEST DIRECTORY