Laboratory：Akiruno
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- gene-related test
- immune-relevant genetic rearrangement tests
- Immunoglobulin H chain JHrearrangement
Laboratory：Akiruno
○Immunoglobulin H chain JHrearrangement
CODE：05433 5
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Immunoglobulin H chain JHrearrangement
cell
2×107cells
cluture container
11-13 Southern Blot HybridizationGene rearrangement not recognizedSouthern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.
other material
COMMENT
￡Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
