Laboratory：Akiruno
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- gene-related test
- immune-relevant genetic rearrangement tests
- Immunoglobulin H chain JHreconstitution
Laboratory：Akiruno
○Immunoglobulin H chain JHreconstitution
CODE：05433 5
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Immunoglobulin H chain JHreconstitution
cell
2×107
cluture container
11-13 Southern Blot HybridizationGene rearrangement not recognizedSouthern blot hybridization
A method in which DNA digested with restriction enzymes is fractionated by electrophoresis, denatured into single-stranded DNA, transferred onto a nylon membrane using capillary action, and hybridized with a target probe to detect the target gene. It is used to analyze abnormal quantitative and qualitative changes in DNA.
other material
COMMENT
Blood/bone marrow fluid/cells: Avoid cryopreservation. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
