TEST DIRECTORY

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Laboratory：Akiruno

○Immunoglobulin H chain JHrearrangement

CODE：05433 5

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Immunoglobulin H chain JHrearrangement
    Blood (EDTA-2Na added)
    7.0
    PN7 Refrigeration
     11-13 Southern Blot Hybridization

    Southern Blot Hybridization
    A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.

     Gene rearrangement not recognized
other material

COMMENT


￡Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
Collect blood in the container shown below, mix well, and preserve refrigerated.

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