Laboratory:Akiruno
- TOP
- chromosomal test
- chromosomal test for leukemia/malignant lymphomas
- IGH-CCND1(IGH-BCL1) t(11;14) translocation
Laboratory:Akiruno
○IGH-CCND1(IGH-BCL1) t(11;14) translocation
CODE:02837 0
-
TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
IGH-CCND1(IGH-BCL1) t(11;14) translocation
lymph nodes
5×5×5mm
H00
7-10 FISH(Various) Band
Culture lymphocytes or bone marrow cells, and fix metaphase cells. This method then stains the chromosomes with a dye to create a striped pattern (band), and analyzes the distribution and density of the band.
G-Banding, which performs Giemsa staining after treatment with trypsin solution, C-Banding, which performs Giemsa staining after treatment with HCI, Ba (OH) 2, 2×SCC, these include Q-Banding, which involves staining with quinacrine mustard and then observing with a fluorescence microscope, and high-precision banding, which uses mitotic images from the end of prophase to the beginning of metaphase, and observes a larger number of bands than usual.
COMMENT
Avoid freezing. Acceptable days are Monday through Friday.
About Submission of Chromosome Testing Submit the specimen on the same day after collection.
Float a 5 x 5 x 5 mm lymph node in the designated container and preserve it refrigerated.
Submit the specimen on the same day after collection.
CONTAINER
H00 旧容器記号 H
保存液入り (容器容量5mL)
内容:RPMI-1640 FBS 硫酸カナマイシン ノボヘパリンNa 炭酸水素Na HEPES
貯蔵方法:凍結
有効期間:色が薄いピンクの状態で使用してください。
(凍結時は淡黄色ですが解凍すると薄いピンク色に戻ります。)