TEST DIRECTORY

RIA (Radio immunoassay)
Radioimmunoassay
A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity.
Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.