Laboratory:Akiruno

Laboratory:Akiruno
CODE:02225 9
FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .
TEST NAME | SPECIMEN | CONTAINER |
---|---|---|
|
bone marrow aspirate
|
CAP COLOR | STORE | TURNAROUND |
---|---|---|
| 7-10 |
METHODOLOGY | REFERENCE RANGE |
---|---|
FISH FISH (Fluorescence in situ hybridization) |
|
Avoid freezing. Acceptable days are Monday through Friday. It detects 5q- using the CSF1 receptor as an indicator.
About Submission of Chromosome Testing Submit the specimen on the same day after collection.
Collect 1.0 mL of bone marrow fluid aseptically in a designated container, mix well, and preserve refrigerated.
Submit the specimen on the same day after collection.
With the preservation solution (Container capability of 5 mL)
Contents: RPMI-1640 FBS Kanamycin sulfate Novo-heparin sodium Sodium bicarbonate HEPES
Storage method: Freeze
Expiration period: Use this after confirming that the solution color has turned to pale pink.
The color turns to pale yellow after freezing and to pale pink after thawing.