TEST DIRECTORY

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Laboratory:Akiruno

CSF1R del(5) long arm deletion

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Specified days
    CSF1R del(5) long arm deletion
    bone marrow aspirate
    1.0
    H00 Refrigeration
    7-10 FISH

    FISH (Fluorescence in situ hybridization)
    Fluorescence in situ hybridization
    Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
    There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

TEST NAME

SPECIMEN
REQUIREMENT
(mL)

CONTAINER

Specified days
CSF1R del(5) long arm deletion

bone marrow aspirate
1.0

CAP COLOR

STORE
TEMPERATURE
(STABILITY)

TURNAROUND
TIME (DAY)

Refrigeration

7-10

METHODOLOGY

REFERENCE RANGE
(UNIT)

FISH

FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

other material

COMMENT


Avoid freezing. Acceptable days are Monday through Friday. It detects 5q- using the CSF1 receptor as an indicator.
About Submission of Chromosome Testing Submit the specimen on the same day after collection.
Collect 1.0 mL of bone marrow fluid aseptically in a designated container, mix well, and preserve refrigerated.
Submit the specimen on the same day after collection.

CONTAINER

With the preservation solution (Container capability of 5 mL)

H00  Previous container symbol H

With the preservation solution (Container capability of 5 mL)

Contents: RPMI-1640 FBS Kanamycin sulfate Novo-heparin sodium Sodium bicarbonate HEPES
Storage method: Freeze
Expiration period: Use this after confirming that the solution color has turned to pale pink.
The color turns to pale yellow after freezing and to pale pink after thawing.

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