CSF1R del(5) long arm deletion

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chromosomal test
chromosomal test for leukemia/malignant lymphomas
CSF1R del(5) long arm deletion

Laboratory：Akiruno

○CSF1R del(5) long arm deletion

CODE：02225 9

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
CSF1R del(5) long arm deletion
bone marrow aspirate
1.0
H00
7-10 FISH
FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
CSF1R del(5) long arm deletion	bone marrow aspirate 1.0	H00

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
		7-10

METHODOLOGY	REFERENCE RANGE (UNIT)
FISH FISH (Fluorescence in situ hybridization) Fluorescence in situ hybridization Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope. There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

METHODOLOGY

REFERENCE RANGE
(UNIT)

FISH

FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
Hybridizes with the target DNA using a probe labeled with a fluorescent dye, and uses the fluorescent site that develops at a specific wavelength as a signal on the chromosome. How to detect under fluorescence microscope.
There is a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which the probe labeled with a labeling substance is bound to the target DNA, and then the labeled probe and fluorescent substance are bound to develop color. .

COMMENT

Avoid freezing. Acceptable days are Monday through Friday. It detects 5q- using the CSF1 receptor as an indicator.
About Submission of Chromosome Testing Submit the specimen on the same day after collection.
Collect 1.0 mL of bone marrow fluid aseptically in a designated container, mix well, and preserve refrigerated.
Submit the specimen on the same day after collection.

CONTAINER

H00 Previous container symbol H

With the preservation solution (Container capability of 5 mL)

Contents: RPMI-1640　FBS　Kanamycin sulfate　Novo-heparin sodium　Sodium bicarbonate　HEPES
Storage method: Freeze
Expiration period: Use this after confirming that the solution color has turned to pale pink.
The color turns to pale yellow after freezing and to pale pink after thawing.

supplementary information

染色体検査で用いられる代表的な記号と略語、核型記載、増殖不良報告について

TEST DIRECTORY