TEST DIRECTORY

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Laboratory：Akiruno

○ML (malignant lymphoma)

CODE：00949 8

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Specified days
    ML (malignant lymphoma)
    bone marrow aspirate
    1.0
    H00 Refrigeration
     8-14 G-band

    (Various) band
    Culture lymphocytes or bone marrow cells, and fix metaphase cells. This method then involves staining the chromosomes with a dye to create striped patterns (bands), and analyzing the distribution and density of the bands.
    G-Banding, Giemsa staining after treatment with trypsin solution; C-Banding, Giemsa staining after treatment with HCI, Ba(OH)2, 2×SCC; Q, staining with quinacrine mustard and observation with a fluorescence microscope. -Banding, a high-precision differential staining method that uses mitotic images from the end of prophase to the beginning of metaphase to increase the number of bands than usual.
other material

COMMENT


Avoid freezing.
The days when we can accept orders (for both blood and bone marrow fluid) are from Monday to Friday.
Please make a request using the name of the disease.
Please see below for target disease names.
If the judgment takes time, the required number of days will be around 20 days.
About submitting a chromosome test　Please submit the specimen on the same day after it is collected.
Notes on Blood Disease Chromosome Testing (G-Banding)
1. Sex information is required to determine G-banding, so please be sure to write your sex on the request form. Also, when requesting G-banding after bone marrow transplantation, please be sure to write the donor sex on the request form.
2. 10 million nucleated cells (1×107 cells) are required for bone marrow chromosome testing. Aseptically collect bone marrow fluid to fill this volume. (This corresponds to 0.1 mL of bone marrow fluid when the number of nucleated cells in the bone marrow is 100,000 cells/μL, and 1 mL of bone marrow fluid when the number of nucleated cells in the bone marrow is 10,000 cells/μL.)
3. Steroid drugs, alkylation During administration of drugs or antimetabolites, chromosomal mitotic figures may not be obtained and testing may not be possible.
4. When requesting peripheral blood, if there are no immature cells (blast) in the blood, or if the blood of a patient in remission cannot be tested because chromosomal mitotic figures cannot be obtained.
Collect 1.0 mL of bone marrow fluid aseptically into the container shown below, mix well, and store refrigerated.
After collecting the specimen, please submit it on the same day.

CONTAINER

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