Laboratory：Akiruno
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- Methotrexate
Laboratory：Akiruno
○Methotrexate
CODE：00790 4
-
TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) BLOOD COLLECTION TIME -
Methotrexate
Plasma
0.5
PH5
↓
A00
2-4 Enzyme immunoassay(EIA)Hazardous limit concentration 24-hour value 10 or moreEIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.
48-hour value 1 or more
72-hour value 0.1 or more (μmol/L) 24, 48, 72 hours during methotrexate/leucovorin rescue therapy
other material
COMMENT
Methotrexate
Points to note concerning blood collecting containers
Avoid using a container with a serum separating agent. It may effect the results on the drug tests.
Points to note concerning the blood collecting time
1. The blood collecting time is for TDM (therapeutic drug monitoring).
2. In repetitive administration, collect a blood sample after a steady state is achieved.
3. When poisoning is developed, the peak concentration also needs to be measured.
4. The blood collecting time shows the standard time: a patient's condition should be taken into account in collecting a blood sample.
After drawing blood into a container shown in the below image, mix well, and separate plasma.
Refrigerate the plasma samples.
CONTAINER
PH5 旧容器記号 G 12
ヘパリン入り (真空採血量5mL)
内容：ヘパリンNa 65IU
貯蔵方法：室温
有効期間：製造から2年
A00 旧容器記号 X
ポリスピッツ
貯蔵方法：室温
