TEST DIRECTORY

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Laboratory:Akiruno

Methotrexate

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
    BLOOD COLLECTION TIME
  • Emergency
    Methotrexate
    Serum
    0.5
    S7P

    A00
    Refrigeration
    2-4 Enzyme immunoassay(EIA)

    EIA (Enzyme immunoassay)
    Enzyme immunoassay
    The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

    Hazardous limit concentration 24-hour value 10 or more
    48-hour value 1 or more
    72-hour value 0.1 or more (μmol/L)
    24, 48, 72 hours during methotrexate/leucovorin rescue therapy
other material

COMMENT


Methotrexate
Points to note concerning blood collecting containers
Avoid using a container with a serum separating agent. It may effect the results on the drug tests.
Points to note concerning the blood collecting time
1. The blood collecting time is for TDM (therapeutic drug monitoring).
2. In repetitive administration, collect a blood sample after a steady state is achieved.
3. When poisoning is developed, the peak concentration also needs to be measured.
4. The blood collecting time shows the standard time: a patient's condition should be taken into account in collecting a blood sample.

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