TEST DIRECTORY

Result interpretation criteria for common bacteria

Test results for items listed below will be reported in the following manner, according to the following SRL criteria:
(Reference: Clinical Microbiology Procedures Handbook - 4th edition)

Standard sets for drug susceptibility testing

• According to the bacterial species (groups) detected, a combination of agents that is effective will be used for testing.
• For those other than the bacterial species (groups) listed above, SRL will select agent(s) for use.
• Please note that the standard patterns of agents may not be used depending on the bacterial species (groups) detected.
• Susceptibility testing is not performed for the normal flora.
• Results of the susceptibility test will be reported using the following categories：
  • S：Susceptible
  • I ：Intermediate
  • R：Resistant

• ▲：VCM will be reported if C. difficile is detected.
• ◎：Testing for N. gonorrhoeae will be performed using disk diffusion method.

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List of dedicated transport containers and transport media

Classification	Material	Specimen volume (mL)	Storage condition	Collection container
Cavity, Airway or Respiratory system	Sputum	2-3		X00	Sterile sputum collection containers
	Throat swab fluid	Appropriate amount		VS1	eSwab105 Regular FLOQ Swab
Gastrointestinal tract	Fecal	Appropriate amount		F01	Cary Blair feces collection tube
	Bile	5-10		ARR	Sterile plastic tube for 10mL
	Gastric fluid	2-3		ARR	Sterile plastic tube for 10mL
Urinary and Reproductive Organs	Midstream urine	5-10		ARR	Sterile plastic tube for 10mL
	Catheter urine	5-10		ARR	Sterile plastic tube for 10mL
	Vaginal secretions	Appropriate amount		VS1	eSwab105 Regular FLOQ Swab
	Pus	Appropriate amount		VS1	eSwab105 Regular FLOQ Swab
Blood and paracentesis fluid	Venous blood	Optimal amount 8-10		CBG CBS	Blood Culture Bottle
	Arterial blood			CBG CBS	Blood Culture Bottle
	Cerebrospinal fluid	2-3		C10	Seed Tube II
	Pleural fluid	5-10		C10	Seed Tube II
	Ascites	5-10		C10	Seed Tube II
	Synovial fluid	5-10		C10	Seed Tube II
Other sites	Otorrhea	Appropriate amount		VS2	eSwab106 Mini Tip FLOQ Swabs
	Eye discharge	Appropriate amount		VS1	eSwab105 Regular FLOQ Swab
	Skin	Appropriate amount		ARR	Sterile plastic tube for 10mL
	Nails	Appropriate amount		ARR	Sterile plastic tube for 10mL
	Tissue	Appropriate amount		ARR	Sterile plastic tube for 10mL
	Pus	Appropriate amount		C10	Seed Tube II
Others	Environmental materials	-		C30	Stamp culture media (for hospital environment test)
		-		VS1	eSwab105 Regular FLOQ Swab
	Gastric biopsy tissue	Appropriate amount		C20	Seed tube HP (for Helicobacter culture identification)
	Fecal	1.0g		F00	fecal container

ARR　Previous container symbol r

Sterile polyethylene test tube

Storage conditions: Room temperature

C10　Previous container symbol n

C20　Previous container symbol n2

C30　Previous container symbol n3

CBG　Previous container symbol m1

CBS　Previous container symbol q1

F00　Previous container symbol U

Stool container

Storage conditions: Room temperature

F01　Previous container symbol d3

VS1　Previous container symbol k

VS2　Previous container symbol k1

X00　Previous container symbol l (Small letter L)

Sterile sputum collection container

Storage conditions: Room temperature

Bacteria test collection method

• Be sure to indicate the name of the material to be tested or the site from which the material was collected.
• If the target organisms are Neisseria gonorrhoeae or Neisseria meningitides, store at .
• Test material for anaerobic culture should be collected in Seed Tube II.
• Note that the number of days required will vary depending on the target bacteria and other factors.

Handling Instructions for Seed Tube II

■ Fluid specimens (paracentesis fluid, digestive fluids, etc.)

1. Sterilize the puncture site (the small circle on the rubber cap) with rubbing alcohol or other sterile cotton.
2. Puncture the syringe needle vertically into the puncture site and inject the fluid specimen.
3. Attach the tube label and transport.
4. Specimens should be tested immediately. If preservation is unavoidable, store at .

■ Solid specimens (pus, feces, tissue fragments, etc.)

1. Sterilize the rubber cap with sterile alcohol cotton, etc.
2. Remove the rubber cap and insert a solid specimen. Insert the specimen all the way into the agar.
   (Note) Cotton swabs up to 7 cm in length can be inserted.
   Anything more than that should be cut off.
3. Quickly and securely close the rubber cap.
4. Attach the tube label and transport.
5. Specimens should be tested immediately. If preservation is unavoidable, store .

Helicobacter culture identification collection method

Handling Instructions for Seed Tube HP

1. Place the collected “gastric biopsy tissue” immediately into the collection container (C20) and insert it below the black line.
2. Specimens should be stored within 30 minutes and requested on the same day.

Environmental Inspection Collection Methods

Hospital environment (wipe method)

Wipe a specific area of the specimen (usually 10cm x 10cm) well with the cotton swab (moistened with medium beforehand) provided with the eSwab105 Regular FLOQ Swab, and re-insert the swab into the medium before submitting it.

Hospital environment (stamp method)

Remove the cap of the stamping medium (C30), and gently press the surface of the medium onto the specimen, being careful not to touch the surface with your fingers. After about 5 seconds, put the cap back on and submit the specimen.