Laboratory：Akiruno
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- gene-related test
- genetic tests for malignant tumor (solid tumor)
- PIK3CA gene mutation analysis (SEQ)（Suspended beyond orders placed 07-04-2024）
Laboratory：Akiruno
○PIK3CA gene mutation analysis (SEQ)（Suspended beyond orders placed 07-04-2024）
CODE：0Y350 8
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
PIK3CA gene mutation analysis (SEQ)（Suspended beyond orders placed 07-04-2024）
Unstained specimen slide *See below
Z10
11-14 Direct sequence methodDirect sequencing method
A method of directly determining the base sequence using DNA amplified by PCR method as a template.
COMMENT
We aim to analyze the mutations of PIK3CA gene exon 9 (E542K, E545K/D) and exon 20 (H1047R) using pathology materials. Only pathology materials can be contracted. Please note in advance that since DNA is fragmented by formalin fixation of tissues, analysis of pathology materials may not be possible depending on the preservation conditions such as the type of fixative solution and fixation time, as well as the preservation period. Duplicate requests of other items are not allowed.
About Unstained Specimen Slide Materials
Please note in advance that since DNA is fragmented by formalin fixation of tissues, analysis of unstained specimen slides may not be possible depending on the preservation conditions such as the type of fixative solution and fixation time, as well as the preservation period.
Please prepare unstained specimen slides, write the section thickness on the front of the slide, place in the object case shown below, preserve and submit at room temperature. When requesting BRAF exon15 V600E (SEQ), PIK3CA gene mutation analysis (SEQ), and c-kit gene mutation analysis (GIST) (either individually or 2 or 3 items at the same time), be sure to request the Tumor site confirmation test (item code No. A910 1) at the same time."
CONTAINER
![[オブジェクトケース] プレパラート (スライドグラス)](../../common/yoki-images/z10.jpg)
Z10 旧容器記号 t 30
[オブジェクトケース]
プレパラート (スライドグラス)
貯蔵方法：室温
supplementary information
BRAF exon15 V600E (SEQ)
c-kit遺伝子変異解析 (GIST) の検査材料
c-kit遺伝子変異解析 (GIST) の検査材料
下記の未染標本スライドを作製し、スライドのおもてに切片の厚さを記入し、 オブジェクトケース (Z10) に入れ、室温保存にてご提出ください。
BRAF exon15 V600E (SEQ)、c-kit遺伝子変異解析 (GIST) 単独または2項目同時の際は、 必ず「腫瘍部位確認用検査」を併せてご依頼くださいますようお願いいたします。
c-kit遺伝子変異解析 (GIST) ご依頼の際は、 必ず「腫瘍部位確認用検査」を併せてご依頼くださいますようお願いいたします。
未染標本スライドの種類と枚数
| 未染標本スライド種類 | 切片の厚さ | 枚数 |
|---|---|---|
| 腫瘍部位確認用 | 3～4μm | 2枚 |
| DNA抽出用 | 10μm | 5～10枚 |
