Laboratory:Akiruno
- TOP
- gene-related test
- genetic tests for malignant tumor (solid tumor)
- BRAFexon15V600E(SEQ)(Suspended beyond orders placed 10-03-2024)
Laboratory:Akiruno
○BRAFexon15V600E(SEQ)(Suspended beyond orders placed 10-03-2024)
CODE:0Y331 9
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TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
BRAFexon15V600E(SEQ)(Suspended beyond orders placed 10-03-2024)
Unstained specimen slide *See below
Z10
11-14 Direct sequence methodDirect sequencing method
A method of directly determining the base sequence using DNA amplified by PCR method as a template.
COMMENT
£We aim to analyze mutations in BRAFV600E in pathology materials. Only pathology materials can be contacted. Please note in advance that since DNA is fragmented by formalin fixation of tissues, analysis of pathology specimens may not be possible depending on the preservation conditions such as the type of fixative solution and fixation time, as well as the preservation period. Please avoid duplicate requests of other items.
About Unstained Specimen Slide Materials
Please note in advance that since DNA is fragmented by formalin fixation of tissues, analysis of unstained specimen slides may not be possible depending on the preservation conditions such as the type of fixative solution and fixation time, as well as the preservation period.
Please prepare an unstained specimen slide, write the section thickness on the front of the slide, place it in the object case shown below, and submit it at room temperature. When requesting BRAF exon15 V600E (SEQ), PIK3CA gene mutation analysis (SEQ), and c-kit gene mutation analysis (GIST) (alone or 2 or 3 items at the same time), please be sure to request them in combination with "Tumor site confirmation test (item code: A9101)."
CONTAINER
Z10 旧容器記号 t 30
[オブジェクトケース]
プレパラート (スライドグラス)
貯蔵方法:室温
supplementary information
BRAF exon15 V600E (SEQ)
PIK3CA 遺伝子変異解析 (SEQ)
c-kit遺伝子変異解析 (GIST) の検査材料
下記の未染標本スライドを作製し、スライドのおもてに切片の厚さを記入し、 オブジェクトケース (Z10) に入れ、室温保存にてご提出ください。
BRAF exon15 V600E (SEQ)、PIK3CA遺伝子変異解析 (SEQ)、 c-kit遺伝子変異解析 (GIST) 3項目のご依頼 (単独または、2~3項目同時の場合) ご依頼の際は、 必ず「腫瘍部位確認用検査」を併せてご依頼くださいますようお願いいたします。
未染標本スライドの種類と枚数
未染標本スライド種類 | 切片の厚さ | 枚数 |
---|---|---|
腫瘍部位確認用 | 3~4μm | 2枚 |
DNA抽出用 | 10μm | 5~10枚 |