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Laboratory：Akiruno

○HLA DNA typing 〔NGS-SBT method〕

CODE：0X186 2

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Specified days
    HLA DNA typing 〔NGS-SBT method〕
    Blood (EDTA-2Na added)
    5.0
    PN5 Refrigeration
     9-20 NGS-SBT method

COMMENT


Avoid freezing.
This item measures HLA gene 11 locus (HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, DPB1).
Consignment days are Monday to Friday. Please be sure to clearly state the relationship, clinical diagnosis name, administered drug name, blood transfusion history, and transplant history. Please avoid duplicate requests with other items.
This test method increases the influence of contamination, so please be careful when handling the sample.
●About HLA samples
1. Be sure to use the specified amount in a special container. Please draw blood. If the amount is unavoidably lower than the specified amount, open the lid and release the negative pressure.
2. We will also use PCR-SSP method and PCR-rSSO method if necessary.
3. If you request HLA typing after transplantation, please note that the alleles of the recipient and donor may be mixed and it may not be possible to determine.
●About types of HLA DNA typing
Please check the latest information on the website of the Japanese Histocompatibility Society below.
https://jshi.smoosy.atlas.jp/ja/hla_data
Collect blood into the container shown below, mix well, and store in the refrigerator. However, in the case of recipients, the number of cells may decrease due to chemotherapy, etc., and DNA may not be collected, so if the recipient's white blood cell count (WBC) is less than 1000/μL, 5.0mL of blood (PN5) is Please submit at least one book. This testing method increases the influence of contamination, so please be careful when handling the specimen.

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