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Laboratory：Akiruno

○MPN gene mutation analysis

CODE：0R625 0

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • MPN gene mutation analysis
    Blood (EDTA-2Na added)
    5.0
    PN5 Refrigeration
    （3 days）
     4-8 PCR

    PCR (Polymerase chain reaction)
    Uses the fact that DNA dissociates from double strands to single strands when heated and returns to double strands when cooled, and binds the desired primer using single stranded DNA as a template. A method in which the target DNA region is exponentially amplified by repeatedly performing DNA synthesis using the transcription reaction of DNA polymerase.

     No mutations detected

COMMENT


Avoid freezing.
Please avoid duplicate requests with other items. This testing method increases the influence of contamination, so please be careful when handling the specimen.
Mutation analysis of JAK2 gene (V617F, exon12), MPL gene (W515L, W515K), and CALR gene (type I to V) The purpose is
If there is a mutation that is not targeted for detection, it may affect the PCR reaction and hybridization reaction. Therefore, accurate judgment results may not be obtained from samples with such mutations.
About submission of hematopoietic tumor genetic testing　Please submit the specimen on the same day after collection.
Collect blood into the container shown below, mix well, and store in the refrigerator.
Please avoid duplicate requests with other items.
This testing method increases the influence of contamination, so please be careful when handling the specimen.

CONTAINER

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