TEST DIRECTORY

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Laboratory：Akiruno

○IDH1/2 gene analysis (glioma) (FFPE)

CODE：0R617 9

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • IDH1/2 gene analysis (glioma) (FFPE)
    Unstained specimen slide
    5-10 pieces 4-5μm
    Z10 Room temperature
     11-14 Direct sequence method

    Direct sequence method
    A method of directly determining the base sequence using DNA amplified by PCR method as a template.

COMMENT


We are analyzing IDH1 R132 and IDH2 R172, which are genetic mutations in glioma. Please note that it is not possible to measure IDH2 gene mutations found in acute myeloid leukemia (AML) patients.
The percentage of tumor cells required for testing is 20% or more. Please see below for points to note when submitting unstained specimen slides.
Please avoid duplicate requests with other items. This testing method can be affected by contamination, so please be careful when handling the specimen.
●Submission conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the proportion of tumor cells present is at least the amount required for the test. If the tumor cell percentage is less than 100%, mark the tumor cell area from the back of the unstained specimen slide.  Please note that if you submit your application without marking, macrodecision may not be possible and this may affect the results, such as false negatives.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks produced within the past 3 years if possible. When thinly slicing, please be careful about contamination by changing the microtome blade for each sample. Additionally, please note that since nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Please be aware of this in advance.

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