DNA histogram（Suspended beyond orders placed 12-03-2020）

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DNA histogram（Suspended beyond orders placed 12-03-2020）

Laboratory：Akiruno

○DNA histogram（Suspended beyond orders placed 12-03-2020）

CODE：08089 5

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
DNA histogram（Suspended beyond orders placed 12-03-2020）
bone marrow aspirate
H00
Please confirm in advance Flow cytometry(FCM)
Flow cytometry
A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed.
When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.
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TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
DNA histogram（Suspended beyond orders placed 12-03-2020）	bone marrow aspirate	H00

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
		Please confirm in advance

METHODOLOGY	REFERENCE RANGE (UNIT)
Flow cytometry(FCM) Flow cytometry A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed. When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.	(%)

METHODOLOGY

REFERENCE RANGE
(UNIT)

Flow cytometry(FCM)

Flow cytometry
A method to analyze individual cells from forward scattered light (cell size), 90° scattered light (internal cell structure) and fluorescence intensity (corresponding antigen on the cell surface) by irradiating cells, which are stained with a monoclonal antibody labeled with a fluorescent dye, with laser light while flowing at high speed.
When performing double staining and analysis using two types of fluorescent dyes, it is called two-color flow cytometry.

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COMMENT

Avoid freezing. Acceptable days are Monday through Friday. No acceptances can be made on the day before public holidays. In the case of paraffin, analysis may be impossible depending on the fixation conditions (at 4°C in 10% formalin, for no more than a few days). For peripheral blood, use two containers (PH5).
Research Test Please note in advance when making requests that, unlike our regularly contracted tests, some of the reference values and clinical significance may not be clear since these tests are performed for the purpose of research.
About Submission of DNA Index and DNA Histogram Submit the peripheral blood and bone marrow fluid specimens on the same day after collection.
Collect the designated amount of blood, mix well, and preserve refrigerated. Submit the specimen on the same day after collection.

CONTAINER

H00 Previous container symbol H

With the preservation solution (Container capability of 5 mL)

Contents: RPMI-1640　FBS　Kanamycin sulfate　Novo-heparin sodium　Sodium bicarbonate　HEPES
Storage method: Freeze
Expiration period: Use this after confirming that the solution color has turned to pale pink.
The color turns to pale yellow after freezing and to pale pink after thawing.

TEST DIRECTORY