TEST DIRECTORY

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Laboratory：Akiruno

○EGFR mutation analysis v2.0

CODE：06582 9

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • EGFR mutation analysis v2.0
    Unstained specimen slide
    5-10 pieces 10μm
    Z10 Room temperature
     3-6 PCR (Real Time PCR)

    Real-time PCR
    It is a type of nucleic acid amplification methods based on PCR method as the basic principle, which is a measurement method that, by using oligonucleotides that emit fluorescence when degraded, enables quantitative of target nucleic acids in real time by confirming the fluorescence signals in each PCR cycle.

COMMENT


The percentage of tumor cells required for testing is 10% or more. Please see below for points to note when submitting unstained specimen slides.
Please avoid duplicate requests with other items.
●Submission conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the proportion of tumor cells present is at least the amount required for the test. If the tumor cell proportion is less than required, mark the tumor cell area from the back of the unstained specimen slide.  Please note that if you submit your application without marking, macrodissection may not be possible and this may affect the results, such as false negatives.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks produced within the past 3 years to the extent possible. When thinly slicing, please be careful about contamination by changing the microtome blade for each sample. Additionally, please note that since nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Please be aware of this in advance.

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