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Laboratory：Akiruno

○BRAF V600 mutation analysis 〔PCR〕

CODE：06520 7

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • BRAF V600 mutation analysis 〔PCR〕
    Unstained specimen slide
    5-10 pieces 10μm
    Z10 Room temperature
     4-10 PCR (Real Time PCR)

    Real-time PCR
    It is a type of nucleic acid amplification methods based on PCR method as the basic principle, which is a measurement method that, by using oligonucleotides that emit fluorescence when degraded, enables quantitative of target nucleic acids in real time by confirming the fluorescence signals in each PCR cycle.

COMMENT


We aim to determine the indication of the BRAF inhibitor (generic name: vemurafenib) treatment of malignant melanoma in pathology materials. The type of mutation in V600 cannot be distinguished. In addition, only pathology materials can be contracted.
The percentage of tumor cells required for the test is 50% or more. Please refer to the following for notes on submitting unstained specimen slides.
Please avoid duplicate requests of other items.
●Submission Conditions
Unstained specimen slides should have been histopathologically evaluated to ensure that the tumor cells are present in at least the required percentage for the test. If the tumor cell percentage is not satisfied, mark the tumor cell area from the back of the unstained specimen slides.   Please note in advance that, if the specimens are submitted without marking, macrodissection cannot be performed, which may affect the results such as a false negative result.
●About Unstained Specimen Slides
The collected tissue should be immediately immersed in a 10% neutral buffered formalin solution for fixation (recommended fixation time: 6-48 hours). When submitting, please prepare serial sections of the designated thickness from formalin-fixed, paraffin-embedded (FFPE) blocks prepared within the last 3 years to the extent possible. Please be careful to avoid contamination by changing the microtome blade for each specimen during thin sectioning, for example. Please note in advance that since DNA is fragmented by formalin fixation of tissues, analysis of unstained specimen slides may not be possible depending on the preservation conditions such as the type of fixative solution and fixation time, as well as the preservation period.
●About Biopsy Specimens
Please note in advance that biopsy specimens are often minute amounts, and the tissue itself may be almost completely lost or may be a piece that contains no tumor cells.

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