BRAF V600 mutation analysis 〔PCR〕

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gene-related test
genetic tests for malignant tumor (solid tumor)
BRAF V600 mutation analysis 〔PCR〕

Laboratory：Akiruno

○BRAF V600 mutation analysis 〔PCR〕

CODE：06520 7

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
BRAF V600 mutation analysis 〔PCR〕
Unstained specimen slide
5 to 10 sheets
thickness 10μm
Z10
4-10 PCR (Real Time PCR)
Real-time PCR
A type of nucleic acid amplification method based on the basic principle of PCR, which uses oligonucleotides that emit fluorescence upon decomposition to quantify target nucleic acids in real time by checking the fluorescent signal at each PCR cycle. A measurement method that enables

COMMENT

The purpose of this test is to determine the applicability of BRAF inhibitor (generic name: vemurafenib) treatment of malignant melanoma (melanoma) in pathology material. The type of V600 mutation cannot be distinguished, and only pathological material can be accepted.
The percentage of tumor cells required for the test is more than 50%.
Please avoid duplicate requests in one sample.
●Submission Conditions
Unstained specimen slides should be histopathologically evaluated to ensure that the required percentage of tumor cells (tumor cells as a percentage of total cells in the specimen) is present. If the required percentage is not met, mark the tumor cell area from the back of the unstained specimen slide. Note that submission of unstained specimen slides without marking may result in failure of macrodissection, which may affect the results, such as a false negative result.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation.
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Be aware of this in advance.

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY