Glucagon (IRG)（Suspended beyond orders placed 03-31-2021）

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endocrinology test
pancreatic hormones and gut hormones
Glucagon (IRG)（Suspended beyond orders placed 03-31-2021）

Laboratory：Akiruno

○Glucagon (IRG)（Suspended beyond orders placed 03-31-2021）

CODE：06465 9

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Glucagon (IRG)（Suspended beyond orders placed 03-31-2021）

Plasma
0.5
PAP
↓
A00
（21 days）
4-10 RIA2 antibody method
RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
70-174(pg/mL)

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
Glucagon (IRG)（Suspended beyond orders placed 03-31-2021）	Plasma 0.5	PAP ↓ A00

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
	（21 days）	4-10

METHODOLOGY	REFERENCE RANGE (UNIT)
RIA2 antibody method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	70-174(pg/mL)

METHODOLOGY

REFERENCE RANGE
(UNIT)

RIA2 antibody method

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

70-174(pg/mL)

COMMENT

Collect blood into the container shown below, mix well, and separate the plasma at low temperature (4℃).
Be sure to store plasma frozen.

CONTAINER

PAP Previous container symbol D

With EDTA-2Na and aprotinin (3mL vacuum blood sampling tube)

Contents: EDTA-2Na 3.75 mg　Aprotinin (1500 units)
Storage method: Room temperature
Expiration period: 1 year from production

A00 Previous container symbol X

Polyethylene test tube

Storage method: Room temperature

TEST DIRECTORY