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Laboratory：Akiruno

○EGFR gene mutation analysis (Scorpion-ARMS method)（Suspended beyond orders placed 07-04-2024）

CODE：06455 0

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • INACTIVE
    EGFR gene mutation analysis (Scorpion-ARMS method)（Suspended beyond orders placed 07-04-2024）
    Unstained specimen slide
    5 to 10 sheets
    thickness 5 to 10?m
    Z10 Room temperature
     4-7 PCR (Real Time PCR)

    Real-time PCR
    A type of nucleic acid amplification method based on the basic principle of PCR, which uses oligonucleotides that emit fluorescence upon decomposition to quantify target nucleic acids in real time by checking the fluorescent signal at each PCR cycle. A measurement method that enables

COMMENT


The percentage of tumor cells required for testing is 10% or more. Please see below for points to note when submitting unstained specimen slides.
Please avoid duplicate requests with other items.
●Submission conditions
Unstained specimen slides must be histopathologically evaluated to ensure that the proportion of tumor cells present is at least the amount required for testing. If the percentage of tumor cells is less than that, mark the tumor cell area from the back of the unstained specimen slide.  Please note that if the sample is submitted without marking, macrodissection may not be possible and the results may be affected, such as false negatives.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution to fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation. .
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Please be aware of this in advance.

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