HTT gene CAG repeat sequence analysis (Huntington's disease)

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gene-related test
genetic tests
HTT gene CAG repeat sequence analysis (Huntington's disease)

Laboratory：Akiruno

○HTT gene CAG repeat sequence analysis (Huntington's disease)

CODE：06415 4

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
HTT gene CAG repeat sequence analysis (Huntington's disease)
Blood (EDTA-2Na added)
2.0
PN2,PN5
（3 days）
12-16 PCR
PCR (Polymerase chain reaction)
Uses the fact that DNA dissociates from double strands to single strands when heated and returns to double strands when cooled, and binds the desired primer using single stranded DNA as a template. A method in which the target DNA region is exponentially amplified by repeatedly performing DNA synthesis using the transcription reaction of DNA polymerase.

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER
HTT gene CAG repeat sequence analysis (Huntington's disease)	Blood (EDTA-2Na added) 2.0	PN2,PN5

CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)
	（3 days）	12-16

METHODOLOGY	REFERENCE RANGE (UNIT)
PCR PCR (Polymerase chain reaction) Uses the fact that DNA dissociates from double strands to single strands when heated and returns to double strands when cooled, and binds the desired primer using single stranded DNA as a template. A method in which the target DNA region is exponentially amplified by repeatedly performing DNA synthesis using the transcription reaction of DNA polymerase.

COMMENT

￡Avoid freezing. This item analyzes the number of CAG repeats in the exon1 region of the HTT gene. Avoid duplicate requests for other items. This testing method can be affected by contamination, so be careful when collecting and handling the specimen.
Ethical guidelines (see below)
Collect blood in the container shown below, mix well, and store in the refrigerator. Avoid duplicate requests for other items. This testing method can be affected by contamination, so be careful when collecting and handling the specimen.

CONTAINER

PN2 Previous container symbol C

With EDTA-2Na (2mL vacuum blood sampling tube)

Contents: EDTA-2Na 3.0 mg
Storage method: Room temperature
Expiration period: 2 years from production

With EDTA-2Na (2 or 5mL vacuum blood sampling tube)

PN5 Previous container symbol C

With EDTA-2Na (5mL vacuum blood sampling tube)

Contents: EDTA-2Na 7.5 mg
Storage method: Room temperature
Expiration period: 2 years from production

TEST DIRECTORY