Laboratory：Akiruno
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- gene-related test
- genetic tests for malignant tumor (solid tumor)
- c-kit gene mutation analysis (GIST)
Laboratory：Akiruno
○c-kit gene mutation analysis (GIST)
CODE：06399 3
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TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
c-kit gene mutation analysis (GIST)
Unstained specimen slide *See below
Z10
11-23 Direct sequence methodDirect sequencing method
A method of directly determining the base sequence using DNA amplified by PCR method as a template.
other material
COMMENT
￡This test is aimed at mutation analysis of c-kit gene exons 9 and 11. Please avoid duplicate requests with other items.
About unstained specimen slide materials
Unstained specimen slides have fragmented DNA due to holmarin fixation of the tissue, so analysis may not be possible depending on the type of fixative, preservation conditions such as fixation time, and the number of years of preservation. [63997] Notes on c-kit gene mutation analysis (GIST).
Submit an unstained specimen slide, write the section thickness on the front of the slide, place it in the object case shown below, and submit it at room temperature.BRAFexon15V600E(SEQ), PIK3CA gene mutation When requesting 3 items (single or 2.3 items at the same time) of analysis (SEQ) and c-kit gene mutation analysis (GIST), be sure to include ″Tumor site confirmation test (item code No. A9101)''
CONTAINER
![[オブジェクトケース] プレパラート (スライドグラス)](../../common/yoki-images/z10.jpg)
Z10 旧容器記号 t 30
[オブジェクトケース]
プレパラート (スライドグラス)
貯蔵方法：室温
supplementary information
BRAF exon15 V600E (SEQ)
PIK3CA 遺伝子変異解析 (SEQ)
c-kit遺伝子変異解析 (GIST) の検査材料
下記の未染標本スライドを作製し、スライドのおもてに切片の厚さを記入し、 オブジェクトケース (Z10) に入れ、室温保存にてご提出ください。
BRAF exon15 V600E (SEQ)、PIK3CA遺伝子変異解析 (SEQ)、 c-kit遺伝子変異解析 (GIST) 3項目のご依頼 (単独または、2～3項目同時の場合) ご依頼の際は、 必ず「腫瘍部位確認用検査」を併せてご依頼くださいますようお願いいたします。
未染標本スライドの種類と枚数
| 未染標本スライド種類 | 切片の厚さ | 枚数 |
|---|---|---|
| 腫瘍部位確認用 | 3～4μm | 2枚 |
| DNA抽出用 | 10μm | 5～10枚 |
