Laboratory:Akiruno

Laboratory:Akiruno
CODE:06399 3
Direct sequencing method
A method of directly determining the base sequence using DNA amplified by PCR method as a template.
£This test is aimed at mutation analysis of c-kit gene exons 9 and 11. Please avoid duplicate requests with other items.
About unstained specimen slide materials
Unstained specimen slides have fragmented DNA due to holmarin fixation of the tissue, so analysis may not be possible depending on the type of fixative, preservation conditions such as fixation time, and the number of years of preservation.
●Notes
It is a prerequisite for requesting that the tissue pieces used for this test must be microscopically examined histopathologically, and the presence of tumor tissue must be confirmed.
Please prepare an unstained specimen slide, write the section thickness on the front of the slide, place it in the object case shown below, and submit it at room temperature. When making a request, please be sure to also include the ”Tumor site confirmation test (item code No. 0A910 0)”.
[オブジェクトケース]
プレパラート (スライドグラス)
貯蔵方法:室温
下記の未染標本スライドを作製し、スライドのおもてに切片の厚さを記入し、 オブジェクトケース (Z10) に入れ、室温保存にてご提出ください。
BRAF exon15 V600E (SEQ)、c-kit遺伝子変異解析 (GIST) 単独または2項目同時の際は、 必ず「腫瘍部位確認用検査」を併せてご依頼くださいますようお願いいたします。
c-kit遺伝子変異解析 (GIST) ご依頼の際は、 必ず「腫瘍部位確認用検査」を併せてご依頼くださいますようお願いいたします。
未染標本スライド種類 | 切片の厚さ | 枚数 |
---|---|---|
腫瘍部位確認用 | 3~4μm | 2枚 |
DNA抽出用 | 10μm | 5~10枚 |