Laboratory：Akiruno
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- gene-related test
- genetic tests for hematopoietic tumor
- major BCR-ABL chimera mRNA quantification（Suspended beyond orders placed 04-02-2018）
Laboratory：Akiruno
○major BCR-ABL chimera mRNA quantification（Suspended beyond orders placed 04-02-2018）
CODE：06392 0
-
TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
major BCR-ABL chimera mRNA quantification（Suspended beyond orders placed 04-02-2018）
bone marrow aspirate
1.0
H00
（1 day）
3-5 RT-PCR (Real Time PCR)No chimeric mRNA detectedReal-time PCR
It is a type of nucleic acid amplification methods based on PCR method as the basic principle, which is a measurement method that, by using oligonucleotides that emit fluorescence when degraded, enables quantitative of target nucleic acids in real time by confirming the fluorescence signals in each PCR cycle.
(copy/μgRNA)
TEST NAME | SPECIMEN | CONTAINER |
|---|---|---|
|
bone marrow aspirate
|
CAP COLOR | STORE | TURNAROUND |
|---|---|---|
| 3-5 |
METHODOLOGY | REFERENCE RANGE |
|---|---|
RT-PCR (Real Time PCR) Real-time PCR |
No chimeric mRNA detected |
COMMENT
Avoid freezing. Acceptable days are Monday through Friday. Please submit the specimen immediately after collection. Please avoid duplicate requests of other items. Since the affects of contamination are greater in this test method, please collect and handle the specimens with care.
About Submission of Leukemia-/malignant lymphoma-related Gene Test Submit the specimen on the same day after collection.
Aseptically collect 1.0 mL of bone marrow fluid in the container shown below, mix well, and preserve refrigerated.
Submit the specimen on the same day after collection.
CONTAINER
H00 Previous container symbol H
With the preservation solution (Container capability of 5 mL)
Contents: RPMI-1640 FBS Kanamycin sulfate Novo-heparin sodium Sodium bicarbonate HEPES
Storage method: Freeze
Expiration period: Use this after confirming that the solution color has turned to pale pink.
The color turns to pale yellow after freezing and to pale pink after thawing.
