TEST DIRECTORY

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Laboratory：Akiruno

○EGFR mutation analysis v2.0 (plasma)

CODE：06350 6

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • EGFR mutation analysis v2.0 (plasma)
    Plasma
    5.0
    PK5,PK7
    ↓
    ARR     Freeze
     3-6 PCR (Real Time PCR)

    Real-time PCR
    A type of nucleic acid amplification method based on the basic principle of PCR, which uses oligonucleotides that emit fluorescence upon decomposition to quantify target nucleic acids in real time by checking the fluorescent signal at each PCR cycle. A measurement method that enables

COMMENT


●About plasma material
Collect a sufficient amount of blood using an EDTA-2K blood collection tube considering the amount of sample to be submitted (5 mL of plasma), and be sure to separate the plasma within 8 hours after blood collection. Immediately after separation, aliquot 2.5 mL of plasma into two sterile polyspits and store frozen. When collecting plasma, do not perform decantation to prevent contamination with genomic DNA derived from leukocyte components. Please avoid duplicate requests with other items. With this testing method, the affects of contamination are greater, so please be careful when handling the sample.
●Notes (from the reagent package insert)
1. In plasma samples, it is possible that EGFR gene mutations cannot be detected even if they are present in the tumor tissue because tumor-derived DNA does not leak sufficiently into the plasma. Therefore, if a plasma test is performed first and a result of an EGFR gene mutation is obtained, consider performing a tissue test if possible.
2.Plasma testing is not a complete replacement for tissue testing.

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