Laboratory:Akiruno
- TOP
- gene-related test
- immune-relevant genetic rearrangement tests
- T cell receptor δ chain Jδ1 rearrangement
Laboratory:Akiruno
○T cell receptor δ chain Jδ1 rearrangement
CODE:06320 9
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TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
T cell receptor δ chain Jδ1 rearrangement
bone marrow aspirate
1.0
H00
11-13 Southern Blot HybridizationGene rearrangement not recognizedSouthern blot hybridization
A method in which DNA digested with restriction enzymes is fractionated by electrophoresis, denatured into single-stranded DNA, transferred onto a nylon membrane using capillary action, and hybridized with a target probe to detect the target gene. It is used to analyze abnormal quantitative and qualitative changes in DNA.
COMMENT
Blood/bone marrow fluid/cells: Avoid cryopreservation. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
Collect 1.0 mL of bone marrow fluid aseptically into the container shown below, mix well, and store refrigerated.
After collecting the specimen, please submit it on the same day.
CONTAINER
H00 旧容器記号 H
保存液入り (容器容量5mL)
内容:RPMI-1640 FBS 硫酸カナマイシン ノボヘパリンNa 炭酸水素Na HEPES
貯蔵方法:凍結
有効期間:色が薄いピンクの状態で使用してください。
(凍結時は淡黄色ですが解凍すると薄いピンク色に戻ります。)