T cell receptor β chain Jβ1 rearrangement

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gene-related test
immune-relevant genetic rearrangement tests
T cell receptor β chain Jβ1 rearrangement

Laboratory：Akiruno

○T cell receptor β chain Jβ1 rearrangement

CODE：06285 9

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
T cell receptor β chain Jβ1 rearrangement
bone marrow aspirate
1.0
H00
11-13 Southern Blot Hybridization
Southern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.
Gene rearrangement not recognized

COMMENT

￡Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
Aseptically collect 1.0 mL of bone marrow fluid in the container shown below, mix well, and preserve refrigerated.
Submit the specimen on the same day after collection.

CONTAINER

H00 旧容器記号 H

保存液入り (容器容量5mL)

内容：RPMI-1640　FBS　硫酸カナマイシン　ノボヘパリンNa　炭酸水素Na　HEPES
貯蔵方法：凍結
有効期間：色が薄いピンクの状態で使用してください。
(凍結時は淡黄色ですが解凍すると薄いピンク色に戻ります｡)

TEST DIRECTORY