Laboratory:Akiruno

Laboratory:Akiruno
CODE:06282 2
Southern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.
TEST NAME | SPECIMEN | CONTAINER |
---|---|---|
T cell receptor γ chain Jγ rearrangement
|
Blood (EDTA-2Na added)
|
CAP COLOR | STORE | TURNAROUND |
---|---|---|
| 11-13 |
METHODOLOGY | REFERENCE RANGE |
---|---|
Southern Blot Hybridization Southern Blot Hybridization | Gene rearrangement not recognized |
£Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
Collect blood in the container shown below, mix well, and preserve refrigerated.
With EDTA-2Na (7mL vacuum blood sampling tube)
Contents: EDTA-2Na 10.5 mg
Storage method: Room temperature
Expiration period: 2 years from production