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Laboratory:Akiruno

T cell receptor γ chain Jγ rearrangement

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
  • T cell receptor γ chain Jγ rearrangement
    Blood (EDTA-2Na added)
    7.0
    PN7 Refrigeration
    11-13 Southern Blot Hybridization

    Southern Blot Hybridization
    A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.

    Gene rearrangement not recognized

TEST NAME

SPECIMEN
REQUIREMENT
(mL)

CONTAINER

T cell receptor γ chain Jγ rearrangement

Blood (EDTA-2Na added)
7.0

CAP COLOR

STORE
TEMPERATURE
(STABILITY)

TURNAROUND
TIME (DAY)

Refrigeration

11-13

METHODOLOGY

REFERENCE RANGE
(UNIT)

Southern Blot Hybridization

Southern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.

Gene rearrangement not recognized

COMMENT


£Blood/bone marrow fluid/cells: Avoid freezing. Please fill in the clinical diagnosis name, etc. on the request form. Please submit the cells stored and refrigerated in a culture container (filled with culture medium) used in the hospital. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization.
Collect blood in the container shown below, mix well, and preserve refrigerated.

CONTAINER

With EDTA-2Na (7mL vacuum blood sampling tube)

PN7  Previous container symbol A

With EDTA-2Na (7mL vacuum blood sampling tube)

Contents: EDTA-2Na 10.5 mg
Storage method: Room temperature
Expiration period: 2 years from production

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