Laboratory:Akiruno
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Laboratory:Akiruno
CODE:06266 0
FISH (Fluorescence in situ hybridization)
Fluorescence in situ hybridization
A method of hybridization with target DNA using a probe labeled with a fluorescent dye, and detection of fluorescent sites that are colored at specific wavelengths as signals on chromosomes under a fluorescence microscope.
There are two methods: a direct method in which a probe labeled with a fluorescent dye is directly bound to the target DNA, and an indirect method in which a probe labeled with a labeling substance is bound to the target DNA, followed by binding of the labeled probe and the fluorescent substance to generate color.